广西鼠伤寒沙门菌1,4,[5],12∶i∶-耐药性及分子分型特征研究  

The drug resistance and molecular characteristics of Salmonella typhimurium 1,4,[5],12∶i∶-in Guangxi

作　　者：曾献莹[1] 吕素玲[1] 瞿聪 蓝兰[1] 谭冬梅[1] 李秀桂[1] ZENG Xianying;LYU Suling;QU Cong;LAN Lan;TAN Dongmei;LI Xiugui(Guangxi Center for Disease Prevention and Control,Guangxi Nanning 530028,China)

机构地区：[1]广西壮族自治区疾病预防控制中心,广西南宁530028

出　　处：《中国食品卫生杂志》2021年第5期558-565,共8页Chinese Journal of Food Hygiene

基　　金：广西壮族自治区卫生健康委员会自筹经费科研课题(Z20190120)。

摘　　要：目的通过研究2018年广西壮族自治区食源性鼠伤寒沙门菌单相变体1,4,[5],12∶i∶-(S.1,4,[5],12∶i∶-)耐药情况和分子分型特征,建立广西壮族自治区食源性S.1,4,[5],12∶i∶-数据库。方法对来自病例监测和食品监测分离的124株S.1,4,[5],12∶i∶-用微量肉汤稀释法进行药敏试验,用脉冲电场凝胶电泳(PFGE)进行分子分型。结果124株S.1,4,[5],12∶i∶-均对亚胺培南敏感,对另外13种抗生素产生不同程度的耐药,对四环素耐药率最高(96.8%,120/124),其次为氨苄西林(95.2%,118/124),三重及以上耐药率为26.6%(33/124)。47.1%S.1,4,[5],12∶i∶-分离株具有氨苄西林、氯霉素、磺胺类、四环素(ACSuT)多重耐药。PFGE结果分为104种带型,相似度为72.9%~100%,优势带型为JPXX01.GX0114(5株)和JPXX01.GX0294(4株),均来源于食源性疾病监测。结论广西壮族自治区S.1,4,[5],12∶i∶-耐药情况不容乐观,特别对青霉素类和四环素类耐药严重。S.1,4,[5],12∶i∶-分子型别呈高度散发状态,及时对S.1,4,[5],12∶i∶-进行分子溯源,可以提高广西壮族自治区在散发病例中识别潜在的食源性S.1,4,[5],12∶i∶-暴发事件的能力。Objective Establishing the database of food-borne Salmonella typhimurium 1,4,[5],12∶i∶-and monophasic variant in Guangxi Zhuang Autonomous Region by investigating the drug resistance and molecular typing characteristics of the isolates in 2018.Methods 124 strains of S.1,4,[5],12∶i∶-from clinic samples and food samples were tested for drug sensitivity by minimum inhibitory concentration(MIC),and molecular typing by pulsed field gel electrophoresis(PFGE).Results One hundred and twenty four strains of S.1,4,[5],12∶i∶-were all sensitive to imipenem,but resistant to the other 13 antibiotics at different levels.The resistance rate to tetracycline was the highest(96.8%,120/124),followed by ampicillin(95.2%,118/124).Multidrug resistant(MDR)rate was 26.6%(33/124),and 47.1%S.1,4,[5],12∶i∶-isolates were multi-resistant to ACSuT.The S.1,4,[5],12∶i∶-strains were differentiated into 104 Xba I PFGE patterns with similarity of 72.9%-100%,and the dominant patterns were JPXX01.GX0114(5 isolates)and JPXX01.GX0294(4 isolates),all of which came from foodborne disease surveillance.Conclusion The result indicated that the S.1,4,[5],12∶i∶-isolates in Guangxi were highly resistant to penicillins and tetracyclines and drug resistance situation was not optimistic,S.1,4,[5],12∶i∶-PFGE pattern were highly sporadic.It is necessary to trace the molecular origin of the isolated strains on time,which can improve ability to identify potential food-borne S.1,4,[5],12∶i∶-outbreak events in sporadic cases.

关 键 词：鼠伤寒沙门菌S.1 4 [5] 12∶i∶- 耐药性 多重耐药 脉冲电场凝胶电泳 分子分型 

分 类 号：R155[医药卫生—营养与食品卫生学]