高脂饮食对小鼠前列腺基因表达的影响  

Impact of high-fat diet on gene expression in mouse prostate tissue

作　　者：郭亚收 徐晓峰 李楠 孙娜 段丽芳 GUO Ya-shou;XU Xiao-feng;LI Nan;SUN Na;DUAN Li-fang(Department of Urology,Xianyang Central Hospital,Xianyang,Shaanxi 712000,China;Faculty of Epidemiology and Health Statistics,School of Public Health,School of Basic Medicine,Shaanxi University of Chinese Medicine,Xianyangy Shaanxi 712046,China;Department of Pathophysiology,School of Basic Medicine,Shaanxi University of Chinese Medicine,Xianyangy Shaanxi 712046,China)

机构地区：[1]咸阳市中心医院泌尿外科,陕西咸阳712000 [2]陕西中医药大学公共卫生学院流行病与卫生统计学教研室,陕西咸阳712046 [3]陕西中医药大学基础医学院病理生理学教研室,陕西咸阳712046

出　　处：《中华男科学杂志》2021年第8期675-679,共5页National Journal of Andrology

基　　金：国家自然科学基金青年项目(81603420);咸阳市2019年科学技术重点研发项目(2019k02-94);咸阳市中心医院2019年院内课题(2019Z07)。

摘　　要：目的:分析高脂饮食对小鼠前列腺组织基因表达芯片数据的生物学网络调控及关键蛋白,为肥胖导致的前列腺癌发生机制提供新的理论依据。方法:从基因芯片公共数据库(GEO)中下载两组C57BL/6J小鼠前列腺组织RNA(正常饮食组5只,高脂肪饮食组4只),利用基因云、基因大数据分析(GCBI)实验平台、GenClip2.0、Sytoscape 3.5.1等分析软件,筛选差异表达基因,探讨差异基因的蛋白交互作用网络和生物学通路,从转录组角度阐述高脂饮食对于前列腺组织基因分子网络改变情况及其可能涉及的分子生物学功能。结果:共筛选出134个差异表达基因,其中上调130个,下调4个,主要涉及染色体组织、细胞-细胞信号传导、小分子生物合成及白细胞激活等生物学功能。基因网络中Lck、Prkcb和Cd28基因Degree值较大,表明其与蛋白质的合成及其生物学功能有很重要的关系,为该蛋白-蛋白网络的核心节点,基Lck和Cd28预测能力较佳。结论:高脂饮食能够诱导前列腺组织基因发生改变,从而导致肿瘤发生。Objective:To analyze the effects of high-fat diet on the biological network regulation of gene expression microarray data and key proteins in mouse prostate tissue,and provide some new theoretical evidence for the mechanism of obesity inducing PCa.Methods:From the Gene Expression Omnibus(GEO),we obtained RNAs in the prostate tissue from two groups of C57 BL/6 J mice,the normal diet group(n=5)and high-fat diet group(n=4).Using the Gene Cloud,Gene-Cloud of Biotechnology Informs(GCBI),GenClip2.0,and Sytoscape 3.5.1,we screened differentially expressed genes,investigated protein interaction networks and biological pathways of differential genes and,from the perspective of transcriptome,explored the effects of high-fat diet on the changes of the molecular network of prostate tissue genes and the molecular biological functions possibly involved.Results:A total of 134 differentially expressed genes were identified,130 up-regulated and 4 down-regulated,mainly involved in biological functions such as chromosome organization,cell-cell signaling,small molecule biosynthesis and leukocyte activation.The Lck,Prkcb and Cd28 genes in the gene network were of high value,indicating an important relationship with protein synthesis and biological functions,the core node of the protein-protein network,and a high predictive ability of Lck and Cd28.Conclusion:The high-fat diet can induce changes in prostate tissue genes,leading to tumorigenesis.

关 键 词：高脂饮食 前列腺组织 基因表达 生物信息学 小鼠 

分 类 号：R697.3[医药卫生—泌尿科学]