高产淀粉酶菌株Bacillus subtilis XC2的产酶条件及酶学性质  

Enzyme-producing conditions and enzymatic properties of amylase producing strain Bacillus subtilis XC2

作　　者：曹慧[1] 张腾月 杨佳萌 张会会 苏奎源 刘真 刘紫祎 张镯 CAO Hui;ZHANG Teng-yue;YANG Jia-meng;ZHANG Hui-hui;SU Kui-yuan;LIU Zhen;LIU Zi-yi;ZHANG Zhuo

机构地区：[1]河南科技大学动物科技学院,河南洛阳471000 [2]广东海洋大学农学院,广东湛江524088

出　　处：《饲料研究》2021年第17期60-64,共5页Feed Research

基　　金：河南科技大学博士科研启动资金(项目编号:13480077);青海省重点研发计划(项目编号:2017-NK-153、2019-NK-173、2019-SF-149)。

摘　　要：试验研究从高产淀粉酶菌株Bacillus subtilis XC2的产酶条件及酶学性质。通过单因素试验和正交试验,初步探究淀粉酶酶学性质并优化菌株发酵产酶条件。结果表明,菌株XC2产淀粉酶的反应最适温度为50℃,最适反应pH值为6.0,在30~50℃和pH值4.0~7.0时酶活稳定性较好,Fe^(2+)、Cu^(2+)和Zn^(2+)对酶活有促进作用,Na^(+)对酶活有抑制作用。菌株XC2产淀粉酶最佳条件为蔗糖添加量1.5%、牛肉膏添加量2.0%、K^(+)添加量0.6%、培养温度37℃、转速200 r/min、初始pH值7.0、发酵时间21 h。研究表明,对产酶条件优化后,Bacillus subtilis XC2产淀粉酶活力达984.5 U/mL。The experiment was to study enzyme-producing conditions and enzymatic properties of amylase producing strain Bacillus subtilis XC2.The enzymatic properties and fermentation condition were conducted by single factor and orthogonal test.The results showed that the enzyme was optimally active at temperature 50℃ and pH value 6.0.The enzyme activity was relative stable under 30~50℃and pH value 4.0~7.0.The amylase activity of XC2 was enhanced by Fe^(2+),Cu^(2+)and Zn^(2+),but reduced by Na^(+).The optimum fermentation conditions were sucrose 1.5%,beef extract 2.0%,K^(+)0.6%,37℃,rotation speed 200 r/min,pH value 7.0,fermentation time 21 h.The experiment indicates that the highest amylase activity of Bacillus subtilis XC2 reached 982.9 U/mL by optimization.

关 键 词：淀粉酶 Bacillus subtilis 酶学性质 产酶条件优化 

分 类 号：S816.3[农业科学—饲料科学]