Er,Cr:YSGG激光清除SLA钛表面牙龈卟啉单胞菌生物膜  

Er,Cr:YSGG laser removal of Porphyromonas gingivalis biofilm on SLA surface

作　　者：余金兰 夏荣[1] 刘春[1] 徐基亮[1] 孙磊[1] Yu Jinlan;Xia Rong;Liu Chun(Dept of Stomatology,The Second Affiliated Hospital of Anhui Medical University,Hefei 230601)

机构地区：[1]安徽医科大学第二附属医院口腔科,合肥230601

出　　处：《安徽医科大学学报》2021年第10期1531-1534,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家重点研发计划项目(编号:2018YFB0407204)。

摘　　要：目的探究应用不同功率Er,Cr:YSGG激光清除喷砂酸蚀(SLA)钛表面牙龈卟啉单胞菌生物膜的效果。方法在SLA试件表面制备牙龈卟啉单胞菌生物膜,并随机分为3组,NL组:不处理;L1组:0.75 W的Er,Cr:YSGG激光处理;L2组:1.5 W的Er,Cr:YSGG激光处理。利用扫描电子显微镜观察各组试件表面细菌生物膜情况,同时应用活/死细菌染色和结晶紫染色法来检测各组试件表面细菌情况。结果扫描电镜和活/死细菌染色在L1、L2组几乎观察不到细菌的存在,但扫描电子显微镜下L1组试件表面仍覆盖大量细菌生物膜。结晶紫染色法的结果显示L1、L2组与NL组OD值差异有统计学意义,但L1、L2之间差异无统计学意义。结论1.5 W、30 Hz的Er,Cr:YSGG激光可以安全有效地清除SLA钛表面牙龈卟啉单胞菌生物膜。Objective To explore the effect of using Er,Cr:YSGG laser with different power to remove the Porphyromonas gingivalis(P.gingivalis)biofilm on sandblasted,large-grit,acid-etched(SLA)surface.Methods P.gingivalis biofilm was prepared on SLA surface and randomly divided into three groups.NL:no treatment;L1:0.75 W Er,Cr:YSGG laser treatment;L2:1.5 W Er,Cr:YSGG laser treatment.Scanning electron microscope(SEM)was used to observe the bacterial biofilm on SLA surface,and live/dead bacterial staining and crystal violet staining methods were used to detect the bacteria in each group.Results Under SEM and live/dead bacterial staining,almost no bacteria was observed in the L1 and L2 groups,but L1 group was still covered with a large amount of acquired pellicle.The results of crystal violet staining showed that L1 and L2 groups were statistically different from NL group,but the difference between the two groups was not statistically significant.Conclusion 1.5 W,30 Hz Er,Cr:YSGG laser can safely and effectively remove the P.gingivalis biofilm on the SLA surface.

关 键 词：Er Cr:YSGG激光 喷砂酸蚀 牙龈卟啉单胞菌 扫描电子显微镜 种植体周围炎 

分 类 号：R781.42[医药卫生—口腔医学] R782.12[医药卫生—临床医学]