壳聚糖硒抗ZEA对仔猪肝肾抗氧化功能、血清E2含量和肝脏3β-HSD活性的影响  

Anti-Zearalenone Effects of Chitosan Selenium on Hepatic and Renal Oxidative Damage, Serum E and 3β-HSD Activity in Weaning Gilts

作　　者：王欢欢 傅春妮 李元辉 赵方红 刘丽娜 秦顺义 马吉飞 WANG Huan-huan;FU Chun-ni;LI Yuan-hui;ZHAO Fang-hong;LIU Li-na;QIN Shun-yi;MA Ji-fei(College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]天津农学院动物科学与动物医学学院,天津西青300384

出　　处：《中国兽医杂志》2021年第6期43-46,52,共5页Chinese Journal of Veterinary Medicine

基　　金：天津市自然科学基金重点项目(20JCZDJC00170);天津市高校“中青年骨干创新人才培养计划”人选资助计划项目;天津市131创新型人才团队建设项目(20180318)。

摘　　要：为研究壳聚糖硒拮抗玉米赤霉烯酮对仔猪肝肾抗氧化功能、血清雌二醇(E2)含量和3β-羟基类固醇脱氢酶(3β-HSD)活性的影响,本试验将24头断奶仔猪随机均分为4组:对照组(C组)饲喂基础日粮;玉米赤霉烯酮攻毒组(ZEA组)饲喂基础日粮+2.0 mg/(kg·bw) ZEA;壳聚糖硒解毒组(ZEA-Se组)饲喂基础日粮+2.0 mg/(kg·bw) ZEA+0.3 mg/(kg·bw)壳聚糖硒(以硒计);壳聚糖硒组(Se组)饲喂基础日粮+0.3 mg/(kg·bw)壳聚糖硒(以硒计),42 d后测定每头仔猪的肝肾抗氧化功能、血清E2含量和肝脏3β-HSD活性。结果表明:ZEA可显著增加肝脏丙二醛(MDA)含量(P<0.05),ZEA组的肝肾总抗氧化能力(T-AOC)、超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性显著或极显著低于C组(P<0.05或P<0.01),ZEA-Se组和Se组MDA含量、T-AOC和T-SOD与C组无显著差异(P>0.05);ZEA组的血清E2含量极显著低于C组(P<0.01),ZEA组肝脏3β-HSD活性显著高于C组(P<0.05),ZEA-Se组和Se组的血清E2含量和肝脏3β-HSD活性与C组无显著差异(P>0.05)。结果表明壳聚糖硒可拮抗ZEA引起的肝肾氧化损伤,发挥抗氧化作用;壳聚糖硒可拮抗ZEA增加血清E2含量并降低仔猪肝脏3β-HSD活性,缓解ZEA对仔猪的毒性作用。This study was conducted to determine the anti-zearalenone(ZEA) effects of chitosan selenium on hepatic and renal oxidative damage, serum E2 and 3β-HSD activity in weaning gilts. Twenty four weaning piglets were randomly assigned into four groups for a 42 day study, and fed basal diets, basal diets supplemented with 2.0 mg/(kg·bw) ZEA, basal diets supplemented with 2.0 mg/(kg·bw) ZEA and 0.3 mg/(kg·bw) selenium as chitosan selenium, basal diets supplemented with 0.3 mg/(kg·bw) selenium as chitosan selenium, respectively. At the end of the trial, renal oxidative damage, serum E2 and 3β-HSD activity were determined. The results showed that compared with control group, MDA content in ZEA groups were significantly increased(P<0.05), GSH-Px activity, T-SOD and T-AOC content in ZEA groups were significantly decreased(P<0.05 or P<0.01);however, no significant differences in MDA content, T-SOD and T-AOC among ZEA-Se group, Se group and control group(P>0.05) were observed;compared with control group, serum E2 content in ZEA groups was decreased(P<0.01) and 3β-HSD activity in ZEA groups was increased(P<0.05), but no significant differences in serum E2 content and 3β-HSD activity among ZEA-Se group, Se group and control group(P>0.05) were noted. These results indicated that chitosan selenium supplementation plays an anti-oxidative role by reducing hepatic and renal oxidative damage, as well as 3β-HSD activity associated with ZEA, and also by increasing serum E2 in weaning piglets.

关 键 词：壳聚糖硒 玉米赤霉烯酮 仔猪 抗氧化功能 雌二醇 3β-HSD活性 

分 类 号：S828[农业科学—畜牧学]