婴幼儿护肤品的微生物检验方法研究  

Analysis on Microbal Test of Baby Skin Care Products

作　　者：吴娇娇 吕婷婷 王皓[2] 王舒 王丹彧 WU Jiaojiao;LYU Tingting;WANG Hao;WANG Shu;WANG Danyu(Jilin City Institute for Food and Drug Control,Jilin Jilin,132000,China)

机构地区：[1]吉林市食品药品检验所,吉林吉林132000 [2]四平市食品药品检验所

出　　处：《质量安全与检验检测》2021年第5期23-26,42,共5页QUALITY SAFETY INSPECTION AND TESTING

摘　　要：为有效检验化妆品抑菌性,完善现行化妆品微生物检验标准,进一步提高微生物检出率,本文选取金黄色葡萄球菌、铜绿假单胞菌、枯草芽孢杆菌、白色念珠菌作为试验菌株,采用常规法、薄膜过滤法、培养基稀释法对婴幼儿护肤品微生物检验方法进行研究。19个样品中,1个样品菌落总数用薄膜过滤法(300 mL/膜)进行检验;1个样品霉菌酵母菌总数用薄膜过滤法(300 mL/膜)进行检验;4个样品采用培养基稀释法(50 mL/管);1个样品采用培养基稀释法(200 mL/管)进行耐热大肠菌群检验;1个样品采用培养基稀释法(190 mL/瓶)进行金黄色葡萄球菌及铜绿假单胞菌检验。其余样品用常规法检查。为保障婴幼儿护肤品的微生物检出率,建议在婴幼儿护肤品微生物检验前进行方法适用性研究。In order to effectively test the Bacteriostasis of cosmetics,improve the current microbial inspection standards of cosmetics and further improve the microbial detection rate,Staphylococcus aureus,Pseudomonas aeruginosa,Bacillus subtilis and Candida albicans were selected as test strains.The microbial inspection methods of infant skin care products were studied by conventional method,membrane filtration method and medium dilution method.In the total 19 cosmetics,Aerobic bacterial count of 1 sample should be tested by membrane filtration(300 mL/membrane),mold and yeast count of 1 sample should be tested by membrane filtration(300 mL/membrane),4 samples were tested by dilution method(50 mL/tube),1 sample was tested by dilution method(200 mL/tube)for thermotolerant coliform bacteria;1 sample should be tested by dilution method(190 mL/bottle)for Staphylococcus aureus and Pseudomonas aeruginosa.The remaining samples can be tested by routine method.In order to ensure the microbiological detection rate of baby skin care products,it is recommended to conduct method suitability test before cosmetic microbiological testing.

关 键 词：化妆品 婴幼儿护肤品 微生物检验 方法学 

分 类 号：TQ658[化学工程—精细化工]