感应草多糖的化学组成与生物活性研究  

Studies on the Chemical Compositions and Biological Activities of Polysaccharides from Biophytum sensitivum

作　　者：廖彭莹 孙雪芹 李务荣 曾维锦 吴家明 杨珍珍 张淼[2] LIAO Peng-ying;SUN Xue-qin;LI Wu-rong;ZENG Wei-jin;WU Jia-ming;YANG Zhen-zhen;ZHANG Miao(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]广西中医药大学广西壮瑶药重点实验室(壮瑶药协同创新中心),广西南宁530200

出　　处：《化学试剂》2021年第11期1580-1585,共6页Chemical Reagents

基　　金：中药学一流学科项目(桂教科研[2018]12号);2019年广西中医药大学引进博士科研启动基金资助项目(2019BS012);广西壮瑶药重点实验室课题子项目(GXZYKF2019-11);2019—2021年广西一流学科建设开放课题项目(2019XK112);广西中医药大学2020年一方制药大学生科技创新课题项目(2020DXS48);广西中医药大学药学院中药学学科青年教师提升计划项目(050170035);广西高等学校千名中青年骨干教师培育计划项目(桂教师范[2019]81号)。

摘　　要：对感应草多糖的化学组成与生物活性进行研究,采用水提醇沉法、脱色脱蛋白透析处理,得到粗多糖、脱色多糖和精制多糖,测定多糖的主成分含量,采用薄层色谱法(TLC)及柱前衍生化-高效液相色谱法(HPLC)分析多糖的单糖组成,测定多糖对DPPH·和ABTS^(+)·的清除能力、还原能力以及对α-葡萄糖苷酶和DPP-Ⅳ酶的体外抑制作用。感应草多糖由5种单糖组成,对DPPH·和ABTS^(+)·均有清除作用,具有还原能力,对α-葡萄糖苷酶和DPP-Ⅳ酶均有抑制作用。相关性分析表明,多糖的糖含量、糖醛酸含量、还原糖含量和蛋白质含量与其抗氧化活性、α-葡萄糖苷酶抑制活性均无显著相关性。感应草多糖具有抗氧化活性和α-葡萄糖苷酶及DPP-Ⅳ酶抑制活性。To study the chemical compositions and the biological activities of polysaccharides from Biophytum sensitivum,the polysaccharides were obtained by water extraction and alcohol precipitation,depigmentation,deproteinization and dialysis.The main chemical contents of polysaccharides were determined.TLC and pre-column HPLC were used to analyze the monosaccharides compositions of the polysaccharides.The DPPH·and ABTS^(+)·scavenging abilities and the reducing power of the polysaccharides were evaluated.Theα-glucosidase and DPP-Ⅳinhibitory activities were also evaluated in vitro.The monosaccharides in the polysaccharides were mannose,rhamnose,glucose,galactose and xylose.The polysaccharides showed scavenging activities against DPPH·and ABTS^(+)·,also possessed reducing capacity.The polysaccharides showed inhibitory activities againstα-glucosidase and DPP-Ⅳenzyme.Correlation analysis showed that the contents of sugar,uronic acid,reducing sugar and protein had no significant correlation with antioxidant activity andα-glucosidase inhibitory activity.The polysaccharides from Biophytum sensitivum showed antioxidant activities and inhibitory effect againstα-glucosidase and DPP-Ⅳenzyme.

关 键 词：感应草 多糖 单糖组成 抗氧化 Α-葡萄糖苷酶 DPP-Ⅳ酶 

分 类 号：R284.2[医药卫生—中药学]