微小RNA-495-3p与尿路上皮癌胚抗原1在甲状腺乳头状癌组织的表达及其临床意义  

Expression and clinical significance of microRNA-495-3p and urothelial carcinoma associated 1 in papillary thyroid carcinoma

作　　者：洪钟萍 张丽婷 吴文艺[1] 林建清[1] 史海鸿[1] 吴鑫泉 余艺煌 丁明骥 邱伟刚 黄种心[3] 邱建龙 Hong Zhongping;Zhang Liting;Wu Wenyi;Lin Jianqing;Shi Haihong;Wu Xinquan;Yu Yihuang;Ding Mingji;Qiu Weigang;Huang Zhongxin;Qiu Jianlong(Department of Thyroid and Breast Surgery,the Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,China;Department of Endocrinology,the 910th Hospital of the People′s Liberation Army,Quanzhou 362000,China;Department of Pathlogy,the Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,China;Department of Pathlogy,the 910th Hospital of the People′s Liberation Army,Quanzhou 362000,China)

机构地区：[1]福建医科大学附属第二临床医学院甲状腺乳腺外科,泉州362000 [2]联勤保障部队第九一〇医院内分泌科,泉州362000 [3]泉州福建医科大学附属第二临床医学院病理科,362000 [4]联勤保障部队第九一〇医院病理科,泉州362000

出　　处：《中华实验外科杂志》2021年第11期2225-2228,共4页Chinese Journal of Experimental Surgery

基　　金：泉州市科技局高层次人才创业创新项目(2017Z017);泉州市科技计划项目(2018N002S);泉州市科技计划项目(2018Z145);福建医科大学科研苗圃基金项目(2016MP11)。

摘　　要：目的探讨微小RNA(miR-495-3p)与尿路上皮癌胚抗原1(UCA1)在甲状腺乳头状癌(PTC)中的作用。方法 2019年9月至2020年4月,福建医科大学附属第二医院甲状腺乳腺外科采集肿瘤标本,将取得的40对PTC组织作为实验组,与之对应40对癌旁正常组织作为对照组。采用实时荧光定量聚合酶链反应(RT-qPCR)检测40对甲状腺乳头状癌组织及癌旁正常组织中miR-495-3p与UCA1的表达水平,并采用Pearson分析法进行相关性分析。在TPC-1中加入miR-495-3p抑制剂,用RT-qPCR检测UCA1的表达,两样本比较采用t检验。结果在PTC组织中miR-495-3p的表达低于癌旁正常组织(0.78±0.11比1.37±0.64,t=5.741,P<0.01),差异有统计学意义,且miR-495-3p的表达水平与肿瘤的淋巴结转移明显相关(0.74±0.07,t=2.187,P<0.05)。在PTC组织中UCA1的表达高于癌旁正常组织(1.04±0.26比0.97±0.16,t=17.88,P<0.05),差异有统计学意义,且UCA1的表达水平与肿瘤的淋巴结转移(1.134±0.174,t=2.254,P<0.05)、腺外侵犯(0.82±0.06,t=0.773,P<0.05)相关。PTC中miR-495-3p与UCA1的表达水平呈负相关(r=-0.371,P<0.05)。抑制TPC-1中miR-495-3p的表达,提高实验组UCA1的表达水平(1.00±0.01比3.25±0.05,t=5.665,P<0.01)。结论 miR-495-3p可负调控UCA1,从而在PTC进展中发挥作用。Objective To investigate the role of microRNA(miR)-495-3p and urothelial carcinoma associated 1(UCA1)in papillary thyroid carcinoma.Methods Real-time fluorescent quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression levels of miR-495-3p and UCA1 in 40 pairs of papillary thyroid carcinoma tissues and normal adjacent tissues,and the correlation analysis was performed by pearson analysis.The miR-495-3p inhibitor was added to TPC-1,and the expression of UCA1 was detected by RT-qPCR,the t test was used to compare the two samples.Results Compared with normal tissues adjacent to cancer,the expression of miR-495-3p was low in papillary thyroid carcinoma tissue(0.78±0.11 vs.1.37±0.64,t=5.741,P<0.01),there was a significant statistical difference.And the expression level of miR-495-3p was negatively correlated with tumor lymph node metastasis(0.74±0.07,t=2.187,P<0.05).Compared with normal tissues adjacent to cancer,UCA1 was highly expressed in papillary thyroid carcinoma tissues(1.04±0.26 vs.0.97±0.16,t=17.88,P<0.05),there was a significant statistical difference,and the expression level of UCA1 It was positively correlated with tumor lymph node metastasis(1.134±0.174,t=2.254,P<0.05)and extraglandular invasion(0.82±0.06,t=0.773,P<0.05).In papillary thyroid carcinoma,the expression level of miR-495-3p and UCA1 was negatively correlated(r=-0.371,P<0.05).Inhibiting the expression of miR-495-3p in TPC-1 will increase the expression level of UCA1 in the experimental group(1.00±0.01 vs.3.25±0.05,t=5.665,P<0.01).Conclusion MiR-495-3p can negatively regulate UCA1,through a certain regulatory mechanism,thereby playing a role in the progression of papillary thyroid carcinoma.

关 键 词：甲状腺乳头状癌 微小RNA 尿路上皮癌胚抗原1 侵袭 凋亡 

分 类 号：R736.1[医药卫生—肿瘤]