镉胁迫下水培杞柳生理特性及镉吸收转运  

Physiological characteristics and Cd absorption and transportation in Salix integra under Cd stress

作　　者：尹泽润 罗宝利 罗锋 戴凤宾 诸葛玉平[1] 娄燕宏[1] 王会[1] 潘红 崔秀敏[1] 范利荣 YIN Zerun;LUO Baoli;LUO Feng;DAI Fengbin;ZHUGE Yuping;LOU Yanhong;WANG Hui;PAN Hong;CUI Xiumin;FAN Lirong(National Engineering Laboratory for Efficient Utilization of Soil Fertilizer Resources/College of Resources and Environment,Shandong Agricultural University,Tai’an 271018,Shandong,China;Junan Agricultural and Rural Bureau,Linyi 276600,Shandong,China;Zoucheng Agricultural and Rural Bureau,Ji’ning 273500,Shandong,China;Zoucheng Improved Seed Breeding Center,Ji’ning 273518,Shandong,China)

机构地区：[1]土肥资源高效利用国家工程实验室/山东农业大学资源与环境学院,山东泰安271018 [2]山东莒南县农业农村局,山东临沂276600 [3]邹城市农业农村局,山东济宁273500 [4]邹城市良种繁育中心,山东济宁273518

出　　处：《草业科学》2021年第10期1900-1909,共10页Pratacultural Science

基　　金：山东省自然科学基金重大基础研究项目(ZR2018ZC02363);国家重点研发计划(2018YFD0800303)。

摘　　要：为探究镉胁迫对杞柳(Salix integra)生长生理及镉吸收转运的影响,进而明确杞柳应用于镉污染土壤修复的潜力。本研究以杞柳幼苗为试验材料,采用水培试验,研究了不同浓度镉胁迫(0、10、20、30、40 mg·L^(−1))下杞柳的生长、根系形态、根系和叶片抗氧化酶活性,以及杞柳对镉的吸收转运特性。结果表明,杞柳具有较强的耐镉性,吸收的镉主要集中于地上部,且以叶片含量最高,茎含量最低;转运系数随镉浓度升高而增加,但富集系数随镉浓度增加而逐渐下降;镉胁迫对杞柳根系构型具有一定的诱导作用,节点数与根尖数显著上升(P<0.05),而对根系生长的影响表现为“低促高抑”,低浓度(10 mg·L^(−1))镉胁迫下杞柳根系总长度与地下部生物量显著上升(P<0.05),高浓度(40 mg·L^(−1))镉胁迫下杞柳根系生物量与根系生长指标均显著下降(P<0.05);镉胁迫诱导杞柳根系与叶片中产生的超氧阴离子活力单位与过氧化氢酶活性上升,显著抑制谷胱甘肽含量与谷胱甘肽还原酶活性(P<0.05),且抑制效果随镉浓度上升而逐渐增加。综上可知,杞柳具有较强的耐镉性和镉富集特性,且通过优化根系构型、激活抗氧化酶促系统缓解自身所受镉胁迫,具有较高的镉污染修复潜力。To evaluate the potential for Cd-polluted soil remediation by Salix integra under Cd stress,plant growth,physiological characteristics,Cd absorption,and transportation characteristics were observed.S.integra seedlings were employed as experiment materials;plant growth,root configuration,physiological responses of roots and leaves,and Cd absorption and transportation were investigated under different Cd stress concentrations(0,10,20,30,40 mg·L^(−1))using hydroponic equipment.The results showed that S.integra has good Cd tolerance,and the absorbed Cd is mainly concentrated in the shoot,and is highest in leaves and lowest in the stem.The transport coefficient increases while the enrichment coefficient gradually decreases with the increase in Cd concentration.Cd stress had a stimulating effect on root architecture,and the number of nodes and root tips increased significantly(P<0.05).In addition,a low level of Cd(10 mg·L^(−1))stress enhanced the biomass and total root length,and a high level of Cd(40 mg·L^(−1))stress decreased root biomass.At the same time,the superoxide anion activity unit and CAT activity in roots and leaves were induced by Cd stress,but GSH content and GR activity were significantly decreased with increasing Cd concentration(P<0.05).In conclusion,S.integra has good tolerance and enrichment characteristics for Cd stress,and has high Cd pollution remediation potential by promoting root growth and activating the antioxidant enzyme system.

关 键 词：杞柳 镉胁迫 根系构型 抗氧化酶 富集系数 转运系数 镉积累 

分 类 号：S564.5[农业科学—作物学] X173[环境科学与工程—环境科学]