胺鲜酯对铬胁迫下白三叶种子萌发特性的影响  

Effects of diethyl aminoethyl hexanoate on seed germination characteristics of white clover under chromium stress

作　　者：王铎 田雨龙 张鸿建 张庆庆 陈柯如 李州[1] WANG Duo;TIAN Yulong;ZHANG Hongjian;ZHANG Qingqing;CHEN Keru;LI Zhou(College of Grassland Science and Technology,Sichuan Agricultural University,Chengdu 611130,Sichuan,China)

机构地区：[1]四川农业大学草业科技学院,四川成都611130

出　　处：《草业科学》2021年第10期1986-1997,共12页Pratacultural Science

基　　金：国家级大学生创新创业训练计划(202010626029)。

摘　　要：本研究以‘海发’白三叶(Trifolium repens‘Haifa’)为供试材料,研究胺鲜酯(diethyl aminoethyl hexanoate,DA-6)浸种预处理对铬(chromium,Cr^(6+))胁迫下白三叶种子萌发耐性的影响。研究表明,5 mmol·L^(−1)的Cr^(6+)溶液显著抑制白三叶种子萌发(P<0.05)。40~60 mmol·L^(−1)的DA-6可显著提高白三叶在铬胁迫下的萌发指标(P<0.05),且在60 mmol·L^(−1)浓度下效果最佳。Cr^(6+)胁迫下,DA-6浸种使种子萌发过程中游离脯氨酸和可溶性糖含量分别提高1.6和3.8倍,细胞渗透势显著降低(P<0.05),同时显著提高Cr^(6+)胁迫下抗氧化酶活性和抗坏血酸–谷胱甘肽循环代谢(P<0.05),从而有效降低Cr^(6+)胁迫导致的氧化伤害,提高细胞膜稳定性。上述结果表明,DA-6浸种可显著提高白三叶种子在Cr^(6+)胁迫下的萌发率及萌发期耐铬性,这与提高渗透调节能力及增强抗氧化防御系统密切相关。White clover(Trifolium repens‘Haifa’)was selected as experimental material,and the effects of soaking seeds in diethyl aminoethyl hexanoate(DA-6)on tolerance of chromium(Cr)stress during germination were studied.The results showed that a 5 mmol·L^(−1) Cr^(6+)solution significantly inhibited seed germination(P<0.05).DA-6 at 40~60 mmol·L^(−1) significantly increased the germination index of white clover under chromium stress(P<0.05),and the greatest effect was achieved at a concentration of 60 mmol·L^(−1).Moreover,free proline and soluble sugar increased by 1.6 and 3.8 times,respectively,and cell osmotic potential was significantly decreased by 178%for seeds soaked in DA-6 during germination under Cr^(6+)stress(P<0.05).The antioxidant enzyme activity and key enzymes involved in the ascorbate-glutathione metabolism cycle of seeds soaked in DA-6 significantly increased(P<0.05),thereby reducing oxidative damage and improving cell membrane stability during germination under Cr^(6+)stress.These results indicate that DA-6 significantly improves the seed germination rate and Cr^(6+)tolerance of white clover during germination and early development,which are closely associated with enhancement of osmotic adjustment and improvement of antioxidant defense.

关 键 词：铬 抗氧化 渗透调节 非生物胁迫 生长 重金属 胺鲜酯 

分 类 号：X173[环境科学与工程—环境科学] S541.2[农业科学—作物学]