利用全基因组关联分析挖掘粳稻直链淀粉含量调控基因  

Identification of regulation genes influencing amylose content in japonica rice using genome-wide association analysis

作　　者：郑洪亮[1,2] 孙世臣[1] 丁国华[1] 王彤彤[1] 赵宏伟[2] 王敬国[2] 刘化龙[2] 韩笑[2] 邹德堂 来永才[3] ZHENG Hongliang;SUN Shichen;DING Guohua;WANG Tongtong;ZHAO Hongwei;WANG Jingguo;LIU Hualong;HAN Xiao;ZOU Detang;LAI Yongcai(Heilongjiang Acamedy of Agricultural Sciences Postdoctoral Programme,Harbin 150086,China;Germplasm Enhancement and Physiology and Ecology of Food Crops in Cold Region,Ministry of Education,Northeast Agricultural University,Harbin 150030,China;Heilongjiang Acamedy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院博士后科研工作站,哈尔滨150086 [2]东北农业大学寒地粮食作物种质创新与生理生态教育部重点实验室,哈尔滨150030 [3]黑龙江省农业科学院,哈尔滨150086

出　　处：《东北农业大学学报》2021年第10期21-31,共11页Journal of Northeast Agricultural University

基　　金：黑龙江省博士后基金项目(LBH-Z16188);东北农业大学“学术骨干”基金资助项目(20XG24);黑龙江省自然科学基金联合引导项目(LH2019C035);黑龙江省“百千万”工程生物育种重大科技专项(2020ZX16B01)。

摘　　要：直链淀粉含量是评价稻米蒸煮食味品质关键指标,挖掘控制该性状QTLs/基因对于改良稻米品质具有重要意义。研究利用295份粳稻种质组成的自然群体为试验材料,测定2019年和2020年稻米直链淀粉含量,结合重测序得到788 396个高质量SNP,利用Tassel 5.0软件混合线性模型(MLM,Q+K)作全基因组关联分析。结果表明,两年共检测到12个与稻米直链淀粉含量相关QTL,分布在水稻第3、4、11和12染色体上,贡献率范围为8.78%~11.62%,其中qAAC4-2和qAAC12-2在两年中重复检测到。进一步针对这两个QTL区间内所有基因作单倍型分析,结合基因注释和前人研究结果,推测LOC_Os04g33640为影响稻米直链淀粉含量新候选基因。Amylose content is a key index to evaluate the cooking and eating quality in rice,and it is important to explore the QTLs/genes controlling this trait for improving rice quality.In this study,295 japonica rice germplasms were used as experimental materials to determine the amylose content in2019 and 2020.Combining with 788396 high-quality SNPs obtained by resequencing,genome-wide association analysis(GWAS)was performed by mixed linear model(MLM)of TASSEL 5.0 software.The results showed that a total of 12 QTLs associated with amylose content were detected on chromosome 3,4,11 and 12 in two consecutive years,and the R^(2) ranged from 8.78%to 11.62%,among which,qAAC4-2 and qAAC12-2 were detected repeatedly in two years.Haplotype analysis of all the genes in the two QTL intervals was further performed.Combining with genes annotation and previous studies,LOC_Os04g33640 was speculated to be a new candidate gene affecting amylose content in rice.

关 键 词：粳稻 直链淀粉含量 GWAS 候选基因 

分 类 号：S511[农业科学—作物学]