粪产碱杆菌的分离鉴定及其生物转化作用  

Isolation, identification and biotransforming of Alcaligenes faecalis

作　　者：李敏 王琦 魏菁 刘继军[4] 高云航[1] LI Min;WANG Qi;WEI Jing;LIU Jijun;GAO Yunhang(College of Animal Science and Technology,Jilin Agricultural University,Changchun,Jilin 130118,China;Jinpu Customs,Dalian,Liaoning 116600,China;Harbin Customs Technology Center,Harbin,Heilongjiang 150028,China;College of Animal Science and Technology,China Agricultural University,Beijing 100083,China)

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]金普海关,辽宁大连116600 [3]哈尔滨海关技术中心,黑龙江哈尔滨150028 [4]中国农业大学动物科学技术学院,北京100083

出　　处：《微生物学通报》2021年第10期3612-3620,共9页Microbiology China

基　　金：国家重点研发计划(2018YFD0501800,2016YFD0501400);新疆自治区重点研发计划(2017BY082);吉林省科技发展计划(20190201296JC);国家现代农业产业技术体系建设专项(CARS-37)。

摘　　要：【背景】硫化氢(H_(2)S)作为畜牧生产过程中释放的一种有毒有害气体,严重危害畜禽和人类的健康,因此降解硫化氢特别是生物氧化法转化硫化氢已成为当前研究热点。【目的】筛选高效硫氧化菌株并研究其生物转化作用。【方法】以长春市某养鸡场采集的新鲜粪便为材料,分离鉴定硫氧化菌株。采用单因素分析法优化其生长条件,研究生物转化效率,检测soxY、soxZ基因m RNA表达水平。【结果】获得一株高效硫氧化菌株JF9,经鉴定为粪产碱杆菌。最佳生长条件:底物浓度0.5 g/L,温度35℃,初始pH 7.0,在此条件下Na_(2)S去除率达94%以上。菌株JF9存在soxY和soxZ基因,其转录水平在硫源诱导前后差异显著(P<0.05)。【结论】分离得到的粪产碱杆菌具有良好的硫化物转化能力,脱硫过程中硫氧化基因高效表达。[Background] Hydrogen sulfide(H_(2)S) is a toxic and harmful gas released in the process of livestock production, which seriously harmed the health of livestock and human beings. Therefore, the degradation of hydrogen sulfide, especially the transformation of hydrogen sulfide by biological oxidation,has become a current research focus. [Objective] In order to screen the highly efficient sulfur-oxidizing strains and study its biotransformation. [Methods] Using fresh feces collected from a chicken farm in Changchun city as the material, sulfur-oxidizing strains were isolated and identified. The growth conditions were optimized by using single-factor analysis, the biotransformation efficiency was studied,and the m RNA expression levels of soxY and soxZ genes were detected. [Results] A highly efficient sulfur-oxidizing strain, JF9, was successfully obtained, which was preliminarily identified as Alcaligenes faecalis. Optimal growth conditions: substrate concentration 0.5 g/L, temperature 35 ℃, initial pH 7.0.Under these conditions, the removal percentage of Na_(2)S was over 94%. The strain JF9 had soxY and soxZ genes, and their transcription levels were greatly induced by sulfur substrates(P<0.05). [Conclusion] The isolated strain Alcaligenes faecalis had a considerable ability to degrade sulfide, and the sulfur-oxidizing genes were highly expressed during the desulfurization process.

关 键 词：粪产碱杆菌 硫化氢 生物转化 SOX基因 

分 类 号：X172[环境科学与工程—环境科学]