DNA methylation changes induced by BDE-209 are related to DNA damage response and germ cell development in GC-2spd  

作　　者：Xiangyang Li Yue Zhang Xiaomin Dong Guiqing Zhou Yujian Sang Leqiang Gao Xianqing Zhou Zhiwei Sun 

机构地区：[1]Department of Toxicology and Hygienic Chemistry,School of Public Health,Capital Medical University,Beijing 100069,China [2]Beijing Key Laboratory of Environmental Toxicology,Capital Medical University,Beijing 100069,China [3]Experimental Center for basic medical teaching,Basic Medical Sciences,Capital Medical University,Beijing 100069,China

出　　处：《Journal of Environmental Sciences》2021年第11期161-170,共10页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.31770441,31971415).

摘　　要：Decabrominated diphenyl ether(BDE-209)is generally utilized in multiple polymer materials as common brominated flame retardant.BDE-209 has been listed as persistent organic pollutants(POPs),which was considered to be reproductive toxin in the environment.But it still remains unclear about the effects of BDE-209 on DNA methylation and the inducedmale reproductive toxicity.Due to the extensive epigenetic regulation in germ line development,we hypothesize that BDE-209 exposure impacts the statue of DNA methylation in spermatocytes in vitro.Therefore,the mouse GC-2spd(GC-2)cells were used for the genome wide DNA methylation analysis after treated with 32μg/mL BDE-209 for 24 hr.The results showed that BDE-209 caused genomic methylation changes with 32,083 differentially methylated CpGs in GC-2 cells,including 16,164(50.38%)hypermethylated and 15,919(49.62%)hypomethylated sites.With integrated analysis ofDNAmethylation data and functional enrichment,we found that BDE-209 might affect the functional transcription in cell growth and sperm development by differential gene methylation.qRT-PCR validation demonstrated the involvement of p53-dependent DNA damage response in the GC-2 cells after BDE-209 exposure.In general,our findings indicated that BDE-209-induced genome wide methylation changes could be interrelated with reproductive dysfunction.This study might provide new insights into the mechanisms of male reproductive toxicity under the environmental exposure to BDE-209.

关 键 词：Decabrominated diphenyl ether (BDE-209) DNA methylation Functional enrichment DNA damage response Germ cell development GC-2spd 

分 类 号：X171.5[环境科学与工程—环境科学]