Crotalaria ferruginea extract attenuates lipopolysaccharide-induced acute lung injury in mice by inhibiting MAPK/NF-κB signaling pathways  

作　　者：Wei Pan Li-Ping Meng Jie Su Zheng-Biao Yang Wei-Feng Du Zhi-Wei Xu Yun-Xiang Chen Sheng Zhang Feng Xie Cong Xu Hong-Zhong Yang Wei-Hong Ge 

机构地区：[1]Department of Pharmacology,College of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China [2]State Key Laboratory of Safety Evaluation for New Drugs,Hangzhou Medical College,Hangzhou 310013,China [3]Department of Surgical Nursing,The First Affiliated Hospital of Hainan Medical University,Haikou 570102,China

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2021年第11期481-490,共10页亚太热带生物医学杂志（英文版）

基　　金：supported by the Natural Science Foundation of Zhejiang province(Grant LQ19H280009);Special Projects of Zhejiang Academy of Medical Sciences(Grant CA1918D-04,CA1903Q-04);Medical Health Science and Technology Project of Zhejiang Provincial Health Commission(Grant 2020384536)。

摘　　要：Objective:To evaluate the anti-inflammatory activity of Crotalaria ferruginea extract(CFE)and its mechanism.Methods:An intratracheal lipopolysaccharide(LPS)instillationinduced acute lung injury(ALI)model was used to study the antiinflammatory activity of CFE in vivo.The LPS-induced shock model was used to analyze the effect of CFE on survival.LPS-stimulated RAW264.7 cell model was used to investigate the anti-inflammatory activity of CFE in vitro and the effects on mitogen-activated protein kinase(MAPK)or nuclear factor-κB(NF-κB)signaling pathways.Results:CFE administration decreased the number of inflammatory cells,reduced the levels of tumor necrosis factor-α(TNF-α),monocyte chemotactic protein-1(MCP-1),interleukin-6(IL-6),and interferon-γ,and diminished protein content in the bronchoalveolar lavage fluid of mice.CFE also reduced lung wet-to-dry weight ratio,myeloperoxidase,and lung tissue pathological injury.CFE preadministration improved the survival rate of mice challenged with a lethal dose of LPS.CFE reduced LPS-activated RAW264.7 cells to produce nitric oxide,TNF-α,MCP-1,and IL-6.Furthermore,CFE inhibited nuclear translocation and phosphorylation of NF-κB P65,extracellular signal-regulated kinase,c-Jun N-terminal kinases,and P38 MAPKs.Conclusions:CFE exhibits potent anti-inflammatory activity in LPS-induced ALI mice,LPS-shock mice,and RAW264.7 cells,and its mechanism may be associated with the inhibition of NF-κB and MAPK signaling pathways.Crotalaria ferruginea may be a useful therapeutic drug for the treatment of ALI and other respiratory inflammations.

关 键 词：Crotalaria ferruginea Acute lung injury CYTOKINE LIPOPOLYSACCHARIDE Nuclear factor-κB Mitogen-activated protein kinase 

分 类 号：R285.5[医药卫生—中药学]