p33^(ING1)、PDGF-A及bFGF在ARC患者晶状体上皮细胞中的表达及临床意义  

Expression and clinical significance of p33^(ING1),PDGF-A and bFGF in lens epithelial cells of patients with age-related cataract

作　　者：郭清 GUO Qing(Chaoju Ophthalmology Hospital,Inner Mongolia,Hohhot,China,010050)

机构地区：[1]内蒙古朝聚眼科医院,内蒙古呼和浩特010050

出　　处：《分子诊断与治疗杂志》2021年第10期1673-1676,共4页Journal of Molecular Diagnostics and Therapy

基　　金：内蒙古自治区卫生和计划生育委员会医疗卫生科研计划项目(201802032)。

摘　　要：目的分析p33^(ING1)、碱性成纤维细胞生长因子(bFGF)及血小板源性生长因子A(PDGF-A在年龄相关性白内障(ARC)患者晶状体上皮细胞中的表达及临床意义。方法选取2018年4月至2019年4月本院收治的67例ARC患者作为ARC组,选取同期来院就诊的70例近视矫正手术者作为对照组。采用连续环形撕囊法取下ARC组和对照组人晶状体中央直径5 mm范围内的前囊膜,比较两组p33^(ING1)、bFGF、PDGF-A mRNA表达水平,测定各组氧化应激因子[丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)],Pearson分析p33^(ING1)、b FGF、PDGF-A mRNA表达水平与氧化应激因子的相关性。结果ARC组p33^(ING1)表达水平低于对照组,PDGF-A及bFGF mRNA表达水平高于对照组,差异均有统计学意义(P<0.05)。ARC组GSH-Px水平低于对照组,MDA水平高于对照组,差异均有统计学意义(P<0.05)。Pearson分析结果显示:ARC患者p33^(ING1)mRNA表达与GSH-Px水平呈正相关关系(P<0.05),与MDA水平呈负相关关系(P<0.05);PDGF-A及bFGF mRNA表达水平与GSH-Px水平呈负相关关系(P<0.05),与MDA水平呈正相关关系(P<0.05)。结论p33^(ING1)、PDGF-A及bFGF mRNA水平在ARC患者晶状体上皮细胞中呈异常表达,三者水平与ARC患者氧化应激呈明显相关性,检测其水平有利于临床病情评估。Objective To analyze the expression and clinical significance of p33^(ING1),basic fibroblast growth factor(bFGF)and platelet derived growth factor-A(PDGF-A)in lens epithelial cells of patients with age-related cataract(ARC).Methods The 67 patients with ARC admitted to this hospital from April 2018 to April 2019 were selected as the ARC group.In the same period,70 patients who came to the hospital for myoporthosis operation were selected as the control group.The continuous circular capsulorhexis was used to take the anterior capsule within 5 mm from the central diameter of the human lens of patients in the ARC group and the control group.The p33^(ING1),bFGF,PDGF-A mRNA expression levels of patients between the two groups were compared.The oxidative stress factors[malondialdehyde(MDA),glutathione peroxidase(GSH-Px)]were measured in two groups.Pearson was used to analyze the correlation between the expression levels of p33^(ING1),bFGF and PDGF-A mRNA and oxidative stress factors.Results The expression level of p33^(ING1)in the ARC group was lower than that in the control group,and the expression levels of PDGF-A and bFGF mRNA were higher than those in the control group.The differences were statistically significant(P<0.05).The level of GSH-Px in the ARC group was lower than that in the control group,and the level of MDAwas higher than that in the control group,the differences were statistically significant(P<0.05).Pearson analysis showed that p33^(ING1)mRNA expression in ARC patients was positively correlated with GSH-Px level(P<0.05),and negatively correlated with MDA level(P<0.05).The PDGF-A and b FGF mRNA expression levels were negatively correlated with GSH-Px level(P<0.05),and positively correlated with MDA level(P<0.05).Conclusion The levels of p33^(ING1),PDGF-A and b FGF mRNA are abnormally expressed in lens epithelial cells of patients with ARC.The levels of the three indicators are significantly correlated with oxidative stress in patients with ARC.The detection of their levels is beneficial to clinical ass

关 键 词：p33^(ING1) BFGF PDGF-A 年龄相关性白内障 晶状体上皮细胞 

分 类 号：R776.1[医药卫生—眼科]