麦冬皂苷B对前列腺癌PC-3细胞增殖、侵袭和迁移的影响  

Effects of Ophiopogonin B on proliferation, invasion and migration of prostate cancer PC-3 cells

作　　者：陈晓康 范连慧 韩起鹏 曹志强 CHEN Xiao-kang;FAN Lian-hui;HAN Qi-peng;CAO Zhi-qiang(Urinary Surgery,General Hospital of Northern Theater Command,Shenyang 110015;Department of Urology,Shengjing Hospital Affiliated with China Medical University,Shenyang 110004,China)

机构地区：[1]北部战区总医院泌尿外科,辽宁沈阳110015 [2]中国医科大学附属盛京医院泌尿外科,辽宁沈阳110004

出　　处：《解剖科学进展》2021年第5期585-587,591,共4页Progress of Anatomical Sciences

基　　金：辽宁省科学技术计划项目(2020JH2/10300159)。

摘　　要：目的探讨麦冬皂苷B对前列腺癌PC-3细胞增殖、侵袭和迁移的影响及可能机制。方法体外培养PC-3细胞,分别以5、10、20μmol/L麦冬皂苷B处理后,MTT实验检测PC-3细胞增殖能力;Transwell实验检测PC-3细胞迁移和侵袭能力;流式细胞术检测PC-3细胞的细胞周期变化及细胞凋亡水平;Western blot检测细胞周期蛋白Dl(cyclin D1)和基质金属蛋白酶-2/9(matrix metalloproteinase-2/9, MMP-2/9)的表达。结果不同浓度麦冬皂苷B处理后,PC-3细胞的细胞增殖能力、迁移和侵袭能力降低(P<0.01);G 0/G 1期细胞比例与细胞凋亡率明显升高(P<0.01);PC-3细胞cyclin D1、MMP-2与MMP-9的表达水平均明显降低(P<0.01)。结论麦冬皂苷B抑制人前列腺癌PC-3细胞增殖、侵袭及迁移,促进凋亡。Objective To investigate the effect and its possible mechanism of Ophiopogonin B on proliferation, invasion and migration of prostate cancer PC-3 cells. Methods PC-3 cells were cultured in vitro, and then treated with 5, 10, 20 μmol/L Ophiopogonin B respectively. The proliferation of PC-3 cells was detected by MTT assay;Transwell assay was used to detect the migration and invasion of PC-3 cells;The cell cycle and apoptosis of PC-3 cells were detected by flow cytometry;The expressions of cyclin D1 and matrix metalloproteinase-2/9(MMP-2/9) were detected by Western blot. Results After treatment with different concentrations of Ophiopogonin B, the proliferation, migration and invasion of PC-3 cells was decreased(P<0.01);The proportion of cells in G0/G1 phase and the rate of apoptosis was increased significantly(P<0.01);The expression of cyclin D1, MMP-2 and MMP-9 in PC-3 cells was decreased significantly(P<0.01). Conclusion Ophiopogonin B inhibited the proliferation, invasion and migration of human prostate cancer PC-3 cells and promotes apoptosis.

关 键 词：麦冬皂苷B 前列腺癌 PC-3细胞 增殖 迁移 侵袭 

分 类 号：R737.25[医药卫生—肿瘤]