FSP改性TiNbTaZr/Zn的表征和成骨诱导活性评价  

Characterization and osteogenic activity of FSP-modified TiNbTaZr/Zn

作　　者：严毅 杨智[1] 王立强[3] 傅远飞[1] YAN Yi;YANG Zhi;WANG Li-qiang;FU Yuan-fei(Department of Prosthodontics,Shanghai Ninth Peopled Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai 200011;Department of Stomatology,Xiangshan Hospital of Traditional Chinese Medicine,Huangpu District,Shanghai 200020;Stale Key Laboratory of Metal Matrix Composites,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔修复科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海200011 [2]上海市黄浦区香山中医医院口腔科,上海200020 [3]上海交通大学材料学院与工程学院,金属基复合材料国家重点实验室,上海200240

出　　处：《上海口腔医学》2021年第5期472-476,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(31971246)。

摘　　要：目的:探讨搅拌摩擦处理加工后的Ti-35Nb-2Ta-3Zr/Zn(TNTZ/Zn)复合材料是否具备诱导成骨的生物活性。方法:通过搅拌摩擦处理,将Zn添加到TNTZ合金表面,设计对照组为TNTZ(未经FSP的TNTZ),实验组为FSP(未加Zn加工的TNTZ)、TNTZ/Zn-0.5(0.5 mm预制孔)和TNTZ/Zn-1(1 mm预制孔)。使用扫描电子显微镜、X线衍射进行表征分析,检测共培养的大鼠骨髓基质干细胞(bone marrow stromal stem cells,BMSCs)的碱性磷酸酶(ALP)活性,实时定量PCR检测ALP活性,COL-1α、OPN和OCN基因的表达。采用SAS 8.2软件包对数据进行统计学分析。结果:TNTZ/Zn-1组表面Zn纳米粒子分布较均匀,直径为70~80 nm。与TNTZ组相比,TNTZ/Zn-0.5和TNTZ/Zn-1组的ALP表达显著上调(P<0.05和P<0.01)。相比于TNTZ组,TNTZ/Zn-0.5和TNTZ/Zn-1组的ALP活性、COL-1α、OPN和OCN基因表达增加,差异有统计学意义(P<0.05)。结论:通过FSP成功将Zn整合到TNTZ合金表面,制备出纳米级微观结构。TNTZ/Zn复合材料可有效诱导成骨,是一种潜在的种植体材料。PURPOSE:To investigate whether Ti-35 Nb-2 Ta-3 Zr/Zn(TNTZ/Zn)composite material processed by friction stir processing has osteogenic activity.METHODS:Zn was added to the surface of TNTZ alloy by friction stir processing.The control group was TNTZ(TNTZ without FSP),the experimental groups was FSP(FSP without Zn),TNTZ/Zn-0.5(0.5 mm pre-made holes)and TNTZ/Zn-1(1 mm pre-made holes).Surface characterization was performed by scanning electron microscope and X-ray diffraction.Alkaline phosphatase(ALP)activity of co-cultured rat bone marrow stromal stem cells(BMSCs)and COL-1α,OPN and OCN gene expression were detected by real-time quantitative PCR.SAS version 8.2 software was used for data analysis.RESULTS:Zn nanoparticles on the surface of the TNTZ/Zn-1 group were distributed homogeneously,with a diameter range of 70-80 nm.Compared with TNTZ group,the activity of ALP in TNTZ/Zn-0.5 and TNTZ/Zn-1 groups was up-regulated(P<0.05 and P<0.01).Compared with TNTZ group,ALP,COL-1α,OPN and OCN gene expression increased in TNTZ/Zn-0.5 and TNTZ/Zn-1 group,and the difference was statistically significant(P<0.05).CONCLUSIONS:Through FSP,Zn can be successfully integrated onto the surface of TNTZ alloy with nano-scale microstructures.TNTZ/Zn composite material can effectively induce bone formation and is a potential implant material.

关 键 词：骨髓基质干细胞 成骨诱导活性 搅拌摩擦处理 

分 类 号：R782.1[医药卫生—口腔医学]