海带多酚的提取分离纯化工艺研究  

Study on extraction and purification of polyphenol from Laminaria japonica

作　　者：张怡评 杨婷 张红红 胡贤陈 晋文慧 方华 洪专 邱远望 黄鸣清[3] ZHANG Yiping;YANG Ting;ZHANG Honghong;HU Xianchen;JIN Wenhui;FANG Hua;HONG Zhuan;QIU Yuanwang;HUANG Mingqing(Technology Innovation Center for Exploitation of Marine Biological Resources,Third Institute of Oceanography,Ministry of Natural Resources,Xiamen 361005,China;Fujian Zhongyi Pharmaceutical Co.,Ltd.,Quanzhou 362712,China;College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350108,China)

机构地区：[1]自然资源部第三海洋研究所,海洋生物资源开发利用工程技术创新中心,福建厦门361005 [2]福建中益制药有限公司,福建泉州362712 [3]福建中医药大学药学院,福建福州350108

出　　处：《药学研究》2021年第10期641-644,651,共5页Journal of Pharmaceutical Research

基　　金：福建省海洋经济发展资金项目(No.2019-2021、FJHJF-L-2021-4);国家重点研发计划项目(No.2016YFF0201104);厦门海洋研究开发院共建经费项目(No.2021-2022);泉州市引进高层次人才团队项目(No.2018CT010)。

摘　　要：目的建立海带多酚的提取及纯化工艺。方法以乙醇浓度、提取时间、提取温度和料液比进行单因素试验,并在单因素试验基础上采用4因素3水平进行正交试验优化提取工艺,同时采用树脂进行纯化工艺优化。结果确定最佳提取工艺为:采用85%乙醇为溶剂,以料液比1∶65,在65℃下搅拌提取1 h。在提取海带粗多酚后采用大孔吸附树脂进行分离纯化研究,筛选出最佳的大孔吸附树脂SZ-3,优化最佳层析条件,获得高纯度海带多酚(80%)。结论本方法简便可行,可用于海带多酚的提取与纯化。Objective To establish the extraction and purification process of Laminaria japonica polyphenols.Methods The Laminaria japonica polyphenol extraction process was investigated by single factor test with ethanol concentration,extraction time,extraction temperature and feed-liquid ratio.The orthogonal experiment was carried out to determine the optimization extraction condition.Then resin was used to optimize the purification process.Results The optimal condition was ethanol concentration 85%,the material solvent ratio 1∶65,under the temperature of 65℃for 1 h.After extracting crude polyphenols from Laminaria japonica,macroporous adsorption resin was used to separate and purify Laminaria japonica polyphenols.The macroporous adsorption resin suitable for purification of Laminaria japonica polyphenols.The Results showed that SZ-3 had got the best purification effect,and the optimum chromatographic conditions were optimized to obtain high purity Laminaria japonica polyphenols(80%).Conclusion The method was simple and feasible,and can be used for the extraction and purification of Laminaria japonica polyphenols.

关 键 词：海带多酚 提取工艺 正交试验 分离纯化 

分 类 号：R284.2[医药卫生—中药学]