N-甲基-N′-硝基-N-亚硝基胍灌胃配合复合因素建立大鼠胃癌模型  

Study on Establishment of Rat Gastric Cancer Model by Intragastric Administration of N-Methyl-N′-Nitro-N-Nitrosoguanidine Combined with Compound Factors

作　　者：李璐璐 陈国忠[2] 吴瑕 袁铁超 王婕 欧智海 易志忠 LI Lu-lu;CHEN Guo-zhong;Wu Xia;YUAN Tie-chao;WANG Jie;OU Zhi-hai;YI Zhi-zhong(Guangxi University of Chinese Medicine,Nanning,Guangxi,China,530000;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning,Guangxi,China,530000)

机构地区：[1]广西中医药大学,广西南宁530000 [2]广西中医药大学第一附属医院,广西南宁530000

出　　处：《河南中医》2021年第12期1846-1850,共5页Henan Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81560758)。

摘　　要：目的:探讨应用N-甲基-N′-硝基-N-亚硝基胍(N-Methyl-N′-Nitro-N-Nitrosoguanidine, MNNG)配合复合因建立大鼠胃癌模型的方法。方法:将50只雄性Wistar大鼠随机分为对照组和实验组,每组25只。对照组给予生理盐水灌胃,实验组以100 mg·L^(-1) MNNG为起始浓度,之后每10周增加25%,直至浓度为150 mg·L^(-1)的递进浓度方式进行灌胃。对照组大鼠普通饲料正常进食,实验组给予高盐饲料并配合饥饱失常(饥1天,饱1天)喂养。从第16周至第40周,每隔4周分别从不同组随机选3只大鼠取胃组织,HE染色观察胃组织病理损伤变化;在20周称大鼠的体质量;统计大鼠的存活率。结果:病理结果显示:实验组大鼠胃组织第24周开始观察到肠化生,28~30周出现不同程度的异型增生,30~40周开始呈现胃癌的进展过程,总诱变率为80.95%;在20周时,与对照组比较,实验组大鼠体质量极显著降低(P<0.01);对照组大鼠的存活率为88.0%,实验组为76.0%。结论:梯度浓度MNNG灌胃配合高盐饲料、饥饱失常等复合因素可建立大鼠胃癌模型。Objective:To investigate the method of establishing gastric cancer model in rats with N-methyl-N′-nitro-N-nitrosoguanidine(MNNG)combined with compound factors.Methods:A total of male Wistar rats were randomly divided into the control group and the experimental group,with 25 rats in each group.The control group was gavaged with normal saline.The experimental group was gavaged with 100 mg·L^(-1) MNNG as the initial concentration,and then increased by 25%every 10 weeks until the concentration was 150 mg·L^(-1).The rats in the control group were fed with normal diet,and the rats in the experimental group were fed with high salt diet combined with abnormal hunger and satiety(hunger for 1 day,satiety for 1 day).From the 16 th week to the 40 th week,three rats were randomly selected from different groups every 4 weeks,and their gastric tissue were removed,then the pathological changes of gastric tissue were observed by HE staining.The body mass of rats was weighed at 20 th week.The survival rate of rats was counted.Results:The pathological results showed that intestinal metaplasia was observed in the gastric tissue of the experimental group from the 24 th week,and different degrees of dysplasia appeared from the 28 th to 30 th week,and the progression of gastric cancer began from the 30 th to 40 th week.The total mutation rate was 80.95%.At 20 th week,compared with the control group,the body mass of rats in the experimental group decreased significantly(P<0.01).The survival rate of the control group was 88.0%,and that of the experimental group was 76.0%.Conclusion:Gradient concentration MNNG combined with high salt diet and abnormal hunger and satiety can establish a good gastric cancer model in rats.

关 键 词：胃癌模型 MNNG 复合因素 大鼠 

分 类 号：R285.5[医药卫生—中药学]