自身免疫性肝炎大鼠血清IL-38和β2GPI水平及其与肝组织病变的关系  

Serum IL-38 and β2GPI levels in rats with autoimmune hepatitis and their relationship with liver tissue lesions

作　　者：史雪丽 张万里 毛俊 颜悦蓉 Shi Xueli;Zhang Wanli;Mao Jun(Clinical Laboratory,Youfu Hospital,Wuhan 430000,Hubei Province,China)

机构地区：[1]武汉市优抚医院检验科,430000 [2]湖北文理学院附属医院,襄阳市中心医院消化内科

出　　处：《实用肝脏病杂志》2021年第6期803-806,共4页Journal of Practical Hepatology

基　　金：湖北省自然科学基金资助项目(编号:WJ2015CB075)。

摘　　要：目的研究自身免疫性肝炎(AIH)大鼠血清白介素-38(IL-38)和β2糖蛋白I(β2GPI)水平及其与肝组织病变的关系。方法在40只大鼠,采用肝细胞特异性脂蛋白联合弗氏完全佐剂腹腔注射法建立AIH大鼠模型,取肝组织行病理学检查,采用ELISA法检测大鼠血清IL-38和β2GPI水平。结果8只重度肝组织病变的AIH大鼠血清IL-38水平为(63.8±9.5)pg/ml,显著低于17只轻度或15只中度AIH大鼠【分别为(100.3±16.9)pg/ml和(85.1±11.3)pg/ml,P<0.05】,而血清β2GPI水平为(4005.9±428.6)μg/L,显著高于轻度或中度AIH大鼠【分别为(2752.1±310.9)μg/L和(3190.5±362.7)μg/L,P<0.05】;6只3级肝组织血管病变的AIH大鼠血清IL-38水平为(39.5±6.9)pg/ml,显著低于18只1级或16只2级血管病变的AIH大鼠【分别为(102.1±17.2)pg/ml和(88.4±11.5)pg/ml,P<0.05】,而血清β2GPI水平为(3866.3±401.7)μg/L,显著高于1级或2级AIH大鼠【分别为(2779.3±309.0)μg/L和(3341.5±370.2)μg/L,P<0.05】。结论随着肝组织血管病变的加重,AIH大鼠血清IL-38水平随之降低,而β2GPI水平随之升高,这两种细胞因子的平衡可能参与了AIH的发病过程。Objective The aim of this study was to serum interleukin-38(IL-38)and β2 glycoprotein I(β2GPI)levels in rats with autoimmune hepatitis(AIH)and their relationship with liver tissue lesions.Methods The AIH model was established by liver-specific lipoprotein and complete Freund’s adjuvant intraperitoneal injection.The liver tissues was obtained for phthological examination,and serum IL-38 and β2GPI levels were detected by ELISA.Results SerumIL-38 level in 8 AIH rats with severe hepatic injuries was(63.8±9.5)pg/ml,significantly lower than in 17 rats with mild or 15 rats with moderate hepatic injuries[(100.3±16.9)pg/ml and(85.1±11.3)pg/ml,respectively,P<0.05],while serumβ2GPI level was(4005.9±428.6)μg/L,significantly higher than in rats with mild or moderate hepatic injuries[(2752.1±310.9)μg/L and(3190.5±362.7)μg/L,respectively,P<0.05];serum IL-38 level in 6 rats with hepatic vascular lesion grade 3 was(39.5±6.9)pg/ml,significantly lower than in 18 rats with grade 1 or 16 rats with grade 2 hepatic vascular lesions[(102.1±17.2)pg/ml and(88.4±11.5)pg/ml,respectively,P<0.05],while serum β2GPI level was(3866.3±401.7)μg/L,much higher than in rats with grade 1 or 2[(2779.3±309.0)μg/L and(3341.5±370.2)μg/L,respectively,P<0.05].Conclusion Serum IL-38 levels decrease,while serumβ2GPI levels increase with the deterioration of liver tissue injuries in rats with AIH,and the balance of the two cytokines might take part in the pathogenesis of AIH,which needs further investigation.

关 键 词：自身免疫性肝炎 白介素-38 β2糖蛋白抗体Ⅰ 肝组织病变 大鼠 

分 类 号：R575.1[医药卫生—消化系统]