利用mRNA-seq和Ribo-seq技术研究低氧条件下SW620细胞的基因表达特征  

The gene expression characteristics of SW620 cells under hypoxia were studied by mRNA-seq and Ribo-seq techniques

作　　者：梅勇 梁绮华 邓载安 汪劲松 MEI Yong;LIANG Qi-hua;DENG Zai-an;WANG Jing-song(Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization,College of Life Sciences,Hubei Normal University,Huangshi 435002,China;School of Health and Environmental Engineering,Shenzhen Technology University,Shenzhen 518118,China)

机构地区：[1]湖北师范大学生命科学学院食用野生植物保育与利用湖北省重点实验室,湖北黄石435002 [2]深圳技术大学健康与环境工程学院,深圳坪山518118

出　　处：《湖北师范大学学报（自然科学版）》2021年第4期30-37,共8页Journal of Hubei Normal University：Natural Science

基　　金：深圳技术大学校企合作项目(编号:2019010802002)。

摘　　要：低氧是结直肠癌肿瘤组织的重要特征和结直肠癌预后不良的重要因素。然而,低氧如何调控直肠癌细胞的基因表达机制仍待深入分析。通过转录组学和翻译组学联合分析揭示了低氧条件下基因表达特征。首先利用化合物CoCl 2处理SW620细胞成功模拟了低氧条件。然后利用mRNA-seq和Ribo-seq分析发现,许多基因的转录和翻译会受低氧条件影响,且多个肿瘤相关的信号通路中的基因翻译效率也受低氧调控。另外,利用real-time PCR和Western blot技术对部分低氧调控的基因表达进行了验证。从新的角度阐明低氧调控肿瘤相关基因表达特征,有助于弄清直结肠癌的发生机制。Hypoxia is an important feature of colorectal cancer and an important factor for poor prognosis of colorectal cancer.However,the mechanism of how hypoxia regulates gene expression in rectal cancer cells remains to be further analyzed.In this study,through combining transcriptomics analysis and translomics analysis,the characteristics of gene expression under hypoxic conditions was revealed.We first treated SW620 cells with the compound cobalt chloride to successfully simulate hypoxia conditions.We then used mRNA-seq and Ribo-seq analyses to show that transcription and translation of many genes are affected by hypoxia,and that the efficiency of gene translation in several tumor-related signaling pathways is also regulated by hypoxia.In addition,real-time PCR and Western blot were used to verify the expression of some hypoxic regulated genes.This study elucidates the expression characteristics of tumor-related genes regulated by hypoxia from a new perspective and helps to clarify the pathogenesis of straight colon cancer.

关 键 词：SW620 低氧 HIF1-α MRNA-SEQ Ribo-seq 

分 类 号：Q7[生物学—分子生物学]