蜡样芽胞杆菌BC307的分离鉴定及其RsiP蛋白的抗体制备  

Identification of Bacillus cereus BC307 and the antibody preparation against RsiP

作　　者：郑庆芳 刘丽娜 刘先凯 吕宇飞 王恒樑[1,2] 王东澍 邱泽武 ZHENG Qingfang;LIU Lina;LIU Xiankai;LYU Yufei;WANG Hengliang;WANG Dongshu;QIU Zewu(College of Food,Shanghai Ocean University,Shanghai 201306,China;State Key Laboratory of Pathogen and Biosecurity,Institute of Biotedchnology,Beijing 100071,China;Xing’an League People’s Hospital,Ulanhot 137400,China;Department of Poisoning Treatment,the Fifth Medical Center of PLA General Hospital,Beijing 100071,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]军事科学院军事医学研究院生物工程研究所病原微生物生物安全国家重点实验室,北京100071 [3]兴安盟人民医院,内蒙古乌兰浩特137400 [4]解放军总医院第五医学中心(南院区)中毒救治科,北京100071

出　　处：《食品与发酵工业》2021年第21期53-58,共6页Food and Fermentation Industries

基　　金：国家自然科学基金项目(81871619)。

摘　　要：蜡样芽胞杆菌(Bacillus cereus)是一种常见的食源性致病菌。从食物中毒病人的食物残渣中分离到1株产呕吐毒素的蜡样芽胞杆菌BC307,其对青霉素类抗生素具有较强的抗性。抗SigP因子RsiP是蜡样芽胞杆菌族(Bacillus cereus sensu lato)细菌青霉素耐药的重要调控因子。该实验利用原核系统表达RsiP蛋白并利用亲和层析法进行纯化,免疫小鼠后获得高效价多克隆抗体。结果表明,RsiP蛋白在炭疽芽胞杆菌、蜡样芽胞杆菌、苏云金芽胞杆菌中高度同源,在Western Blotting实验中RsiP抗血清与这3种菌的全菌蛋白均能发生特异性免疫反应。研究结果为探究RsiP对蜡样芽胞杆菌群细菌的青霉素耐药性调控打下了良好的基础。Bacillus cereus is a common food-borne pathogen and anti-SigP factor RsiP is an important regulator of penicillin resistance.B.cereus BC307 strain from the food residues of food poisoning patient was isolated,which has strong resistance to penicillin.The RsiP protein was expressed and purified by affinity chromatography.High-titer polyclonal antibody was obtained after immunizing mice.The results showed that the RsiP protein was highly conserved and identical in B.anthracis,B.cereus,and B.thuringiensis.Western blotting showed that the RsiP antiserum had specific immunological reactions with the whole bacterial protein of these three bacteria.The studies lay a good foundation for exploring the regulation of penicillin resistance of B.cereus by RsiP.

关 键 词：蜡样芽胞杆菌 分离鉴定 青霉素耐药 RSIP 抗体制备 

分 类 号：TS201.3[轻工技术与工程—食品科学]