油茶籽粕多糖羧甲基化、乙酰化修饰及其对透明质酸酶的抑制作用  

Study on carboxymethylated and acetylated modification process of Camellia Oleifera seed meal polysaccharides and its hypoglycemic activity

作　　者：杨建安 张超 文焱炳 方芳 YANG Jian-an;ZHANG Chao;WEN Yan-bing;FANG Fang(Guizhou Research Institute of Chemical Engineering,Guiyang,Guizhou 550002,China;School of Chemistry and Food Engineering,Changsha University of Science and Technology,Changsha,Hunan 410114,China)

机构地区：[1]贵州省化工研究院,贵州贵阳550002 [2]长沙理工大学化学与食品工程学院,湖南长沙410114

出　　处：《食品与机械》2021年第10期44-49,共6页Food and Machinery

基　　金：贵州省科学技术基金重点项目(编号:黔科合基础[2017]1414);贵州省科技计划项目(编号:黔科合支撑[2020]1Y143号);湖南省教育厅优秀青年项目(编号:19B015);湖南省重点研发项目(编号:2020SK2100)。

摘　　要：目的:研究羧甲基化油茶籽粕多糖(CM-COP)和乙酰化油茶籽粕多糖(Ac-COP)的修饰工艺,并研究其对透明质酸酶的抑制作用。方法:以取代度为指标,采用氢氧化钠—氯乙酸法和乙酸酐法分别对油茶籽粕多糖(COP)进行羧甲基化和乙酰化修饰。结果:CM-COP最佳修饰工艺为氢氧化钠浓度1.5 mol/L,反应时间3 h,反应温度60℃;Ac-COP最佳修饰工艺为乙酸酐用量3.0 mL,反应时间3 h,反应温度60℃;COP、CM-COP、Ac-COP对透明质酸酶的抑制率分别为78.0%,90.3%,92.2%。结论:COP、CM-COP、Ac-COP对透明质酸酶均具抑制作用,且CM-COP和Ac-COP的抑制活性高于COP,说明改性修饰能增加COP的生物活性。Objective:To study the modification process of carboxymethylated Camellia Oleifera seed meal polysaccharide(CM-COP)and acetylated Camellia Oleifera seed meal polysaccharide(Ac-COP),and to study their inhibitory effects on hyaluronidase.Methods:Using the degree of substitution as an index,the carboxymethylation and acetylation modification of Camellia Oleifera seed meal polysaccharide(COP)were carried out by sodium hydroxide-chloroacetic acid method and acetic anhydride method,respectively.Results:The best modification process for CM-COP was:sodium hydroxide concentration of 1.5 mol/L,reaction time of 3 h,reaction temperature of 60℃;Ac-COP best modification process is:acetic anhydride dosage 3.0 mL,reaction time 3 h,The reaction temperature was 60℃;and the inhibition rates of COP,CM-COP,and Ac-COP to hyaluronidase were 78.0%,90.3%and 92.2%,respectively.Conclusion:COP,CM-COP,and Ac-COP all have inhibitory effects on hyaluronidase,and the inhibitory activities of CM-COP and Ac-COP are higher than COP,indicating that the modification can increase the biological activity of COP.

关 键 词：油茶籽粕 多糖 羧甲基化:乙酰化 透明质酸酶 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]