新型矿化胶原膜的体外细胞相容性评价  

In vitro cytocompatibility evaluation of a novel mineralized collagen membrane

作　　者：韩雪 许亦权 李大伟 赵海丹 刘振 Han Xue;Xu Yiquan;Li Dawei;Zhao Haidan;Liu Zhen(Department of Stomatology,The Eighth Medical Center of Chinese PLA General Hospital,Beijing 100091;Dept of Orthopedics,The Eighth Medical Center of Chinese PLA General Hospital,Beijing 100091)

机构地区：[1]中国人民解放军总医院第八医学中心口腔科,北京100091 [2]中国人民解放军总医院第八医学中心骨科,北京100091

出　　处：《口腔材料器械杂志》2021年第4期225-229,共5页Chinese Journal of Dental Materials and Devices

基　　金：中国人民解放军总医院军事医学转化项目(编号:ZH19028);军队医学科技青年培育项目(编号:20QNPY109)。

摘　　要：目的观察新型矿化胶原膜对MG63成骨样细胞体外细胞相容性的影响。方法采用体外仿生矿化技术构建新型矿化胶原双层膜,致密层为Ⅰ型胶原,疏松层为纳米羟基磷灰石-胶原。进行HE染色和扫描电镜(SEM)观察MG63细胞在新型矿化胶原膜表面的生长和黏附情况;以新型矿化膜浸提液培养MG63细胞,另设空白对照组,检测MG63细胞的细胞周期和凋亡率。采用SPSS 17.0软件对数据进行统计学分析。结果HE染色可见大量细胞附着在矿化胶原膜表面;SEM下观察细胞在胶原膜上生长良好,通过突起与粗糙表面紧密接触;细胞周期检测显示,对照组细胞的G_(0)/G_(1)期、S期、G_(2)/M期比率分别为(74.05±0.45)%、(17.03±1.34)%、(8.93±1.02)%,矿化胶原膜组为(75.11±0.97)%、(16.32±0.89)%、(8.13±0.46)%,差异无统计学意义(P>0.05);对照组细胞的凋亡率为(4.25±1.26)%,矿化胶原膜组为(4.27±0.36)%,差异也无统计学意义(P>0.05)。结论矿化胶原膜对MG63细胞生长无抑制作用,具有良好的生物学性能。Objective The study aimed to evaluate the effects of a new mineralized collagen membrane on in-vitro cytocompatibility of MG63 osteoblasts.Methods In vitro biomimetic technology is used to construct a new mineralized collagen bilayer membrane,the dense layer is collagenⅠ,loose layer is nano hydroxyapatite-collagen..HE staining and scanning electron microscopy(SEM)were used to observe the MG63 cell growth and adhesion on the surface of the new mineralized collagen membrane,MG63 cells were cultured with the new mineralized membrane extract,and a blank control group was set up to detect the cell cycle and apoptosis of MG63 cells.Statistical analysis was assessed using SPSS17.0 software.Results HE staining showed that a large number of cells were attached to the surface of the mineralized collagen membrane.Cells were observed to grow well on the membrane under SEM,and the protrusions were in close contact with the rough surface.The ratios of G_(0)/G_(1),S,and G_(2)/M phases in the control group were(74.05±0.45)%,(17.03±1.34)%,(8.93±1.02)%,and mineralized collagen membrane group was(75.11±0.97)%,(16.32±0.89)%,(8.13±0.46)%.The apoptosis rate of the control group was(4.25±1.26)%and the collagen membrane group was(4.27±0.36)%.Compared with the control group,there was no statistically significant difference in the cell cycle(P>0.05)and apoptosis rate(P>0.05)of MG63.Conclusion The mineralized collagen membrane has no inhibitory effect on the growth of MG63 cells and it has good biological properties.

关 键 词：胶原膜 MG63 细胞 细胞周期 细胞凋亡 

分 类 号：R783.1[医药卫生—口腔医学]