二维液相色谱-串联质谱法测定婴幼儿奶粉中的壬基酚  

Determination of Nonylphenol Residues in Infant Formula by 2D-HPLC-MS/MS

作　　者：杨晋青[1] 虞成华[1] 翁史昱[1] YANG Jinqing;YU Chenghua;WENG Shiyu(Shanghai Institute of Quality Inspection and Technical Research,Shanghai 200233,China)

机构地区：[1]上海市质量监督检验技术研究院,上海200233

出　　处：《中国乳品工业》2021年第10期49-53,共5页China Dairy Industry

基　　金：上海市食品质量安全检测与评价专业技术服务平台(18DZ2292400);国家重点研发计划课题(2019YFC1606404)。

摘　　要：建立了一种使用二维液相色谱-串联质谱法检测婴幼儿奶粉中壬基酚的方法。样品经乙腈提取、上清液经过二维液相色谱分离后,采用电喷雾串联质谱检测,内标法定量。对检测方法进行方法验证,线性范围为0.5~50 ng/mL,相关系数≥0.999,方法定量限为1.0μg/kg,高、中、低3个水平的加标回收率为85.4%~97.3%,相对标准偏差为2.08%~5.11%。该方法结果准确、重复性好、操作简单,适用于婴幼儿奶粉基质中壬基酚的测定。2-Dimensional High Performance Liquid Chromatography-Tandem Mass Spectrometry(2 D-HPLC-MS/MS)method was developed to determine nonylphenol in infant formula.Sample was extracted by acetonitrile and concentrated,then was separated by the2 D-HPLC system and determined by ESI-MS-MS,quantified by internal standard method.A linear calibration curve was obtained in the concentration range of 0.5-50 ng/mL.The linear correlation cofficients of the method are above 0.999.The limits of quantifiation(LOQ)was 1.0μg/kg.The recoveries in three levels were ranged from 85.4%-97.3%and RSD was ranged from 2.08%-5.11%.This method was accurate and simple to determine nonylphenol in infant formula.

关 键 词：二维高效液相色谱 串联质谱 婴儿配方奶粉 壬基酚 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]