Abnormal meiosis in fertile and sterile triploid cyprinid fish  

作　　者：Chun Zhang Qi Li La Zhu Wangchao He Conghui Yang Hui Zhang Yu Sun Luojing Zhou Yuandong Sun Shurun Zhu Chang Wu Min Tao Yi Zhou Rurong Zhao Chenchen Tang Shaojun Liu 

机构地区：[1]State Key Laboratory of Developmental Biology of Freshwater Fish,College of Life Sciences,Hunan Normal University,Changsha 410081,China [2]School of Life Sciences,Hunan University of Science and Technology,Xiangtan 411201,China

出　　处：《Science China(Life Sciences)》2021年第11期1917-1928,共12页中国科学（生命科学英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(31873038,31730098 and U19A2040);the earmarked fund for the China Agriculture Research System(CARS45);Key Research and Development Program of Hunan Province(2017NK1031);111 project(D20007).

摘　　要：Meiosis is the key process for producing mature gametes. A natural fertile triploid Carassius auratus population(3nDTCC) and an artificially derived sterile triploid crucian carp(3nCC) have been previously observed, providing suitable model organisms for investigating meiosis characteristics in triploid fish. In the present study, the microstructures and ultrastructures of spermatogenesis were studied in these fishes. Td T-mediated d UTP nick end labeling detection was performed to investigate the apoptosis of spermatocytes. Fluorescence in situ hybridization was employed to trace chromatin pairing. In addition, the m RNA expressions of cell cycle-related genes(i.e., cell division control 2 and cell cycle protein B) were determined by quantitative realtime polymerase chain reaction to illustrate the molecular mechanism of abnormal meiosis in the 3nCC. The results showed that the 3nCC undergoes an irregular prophase I, with the chromosomes distributed in a unipolar radial manner and exhibiting partial pairing, hindered metaphase I, and degenerated cells in the subsequent stages. Meanwhile, the 3nDTCC presented a relatively regular meiotic prophase I with complete conjugate chromosome pairs and chromosomes distributed along the karyotheca,which were presented as a ring structure by slicing. Only the spreads with 130–150 irregular chromosomes can be easily detected in the 3nDTCC, suggesting that it may undergo an abnormal metaphase I. This study provides new insights into the meiosis of fertile and sterile triploid cyprinid fish.

关 键 词：TRIPLOID meiotic configuration partial pairing TELOMERE degenerated cells 

分 类 号：S917.4[农业科学—水产科学]