黄芪多糖对阿尔茨海默病大鼠神经细胞活性、认知功能及Caspase-9表达水平的影响  

Effect of Astragalus polysaccharide on neuronal activity, cognitive function and Caspase-9 expression in Alzheimer’s disease rats

作　　者：屈文英[1] 解建国[1] 梁安心 尚雪峰 Qu Wenying;Xie Jianguo;Liang Anxin(Department of Neurology,Affiliated Hospital of Yan’an University,Yan’an Shaanxi 716000)

机构地区：[1]陕西省延安大学附属医院神经内科,716000

出　　处：《卒中与神经疾病》2021年第5期543-549,共7页Stroke and Nervous Diseases

摘　　要：目的探讨黄芪多糖(Astragalus polysacharin, APS)对阿尔茨海默病(Alzheimer’s disease, AD)大鼠神经细胞活性、认知功能及天冬氨酸特异性半胱氨酸蛋白酶(Cysteinyl aspartate specific protease, Caspase)-9表达水平的影响。方法 36只无特定病原体(Specific pathogen free, SPF)级美国斯泼累格·多雷(Sprague Dawley)雌雄各半的大鼠,按照随机数字表分为6组,正常组、AD组、药物对照组、干预A组、干预B组及干预C组;除正常组外,其余大鼠建立AD大鼠模型;建模后药物对照组采用0.5 g/kg的吡拉西坦灌胃,干预A组、干预B组及干预C祖均采用0.2、0.4及0.8 g/kg的黄芪多糖(Astragalus polysacharin, APS)灌胃,正常组及AD组灌胃等剂量的生理盐水,均1次/d,灌胃时间连续60 d。采用Morris水迷宫实验观察认知功能;苏木精-伊红(Hematoxy lin-Eosin, HE)染色观察海马组织病理学表现;末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法[Terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling, TUNEL]法检测神经细胞凋亡率;免疫印迹及实时定量聚合酶链反应(Quantitatie real time polymerase chain reaction, QRT-PCR)技术分别检测细胞色素C(Cytochrome c, Cyt-c),Caspase-3及Caspase-9蛋白及信使核糖核酸(Messenger RNA,mRNA)相对表达水平。结果与正常组比较,AD组大鼠灌胃后第1~5 d的逃避潜伏期均延长(P<0.05);与AD组比较,干预A组、干预B组、干预C组逃避潜伏期均缩短(P<0.05),药物对照组逃避潜伏期与干预C组比较无明显差异(P>0.05)。与正常组比较,AD组大鼠游泳距离增加(P<0.05);与AD组比较,干预A组、干预B组及干预C组大鼠游泳距离均减少(P<0.05),干预C组与药物对照组相似(P>0.05)。正常组海马结构完成,神经元排列紧密,细胞核清晰且无空泡;AD组大鼠海马组织结构紊乱,神经元数目减少,细胞核深染,细胞膜收缩及部分消失;APS干预组及药物对照组神经元排列较AD组整齐有序,肿胀Objective To explore the effect of Astragalus polysacharin(APS) on neuronal activity, cognitive function and cysteinyl aspartate specific protease-9(caspase-9) expressionin Alzheimer’s disease(AD) rats.Methods 36 specific pathogen-free(SPF) Sprague Dawley male and female rats were randomly divided into 6 groups including normal group, AD group, drug control group, intervention group A, intervention group B and intervention group C. Except the normal group, AD rat model was established in other groups. After modeling, the drug control group was gavaged with 0.5 g/kg piracetam. The intervention group A, intervention group B and intervention group C were gavaged with 0.2, 0.4 and 0.8 g/kg APS, respectively. The normal group and the AD group were gavaged with equal dose of normal salineonce a day. The gavage was performed continuously for 60 days. Morris water maze test was used to evaluate cognitive function. Hematoxy lin-eosin(HE) staining was performed to observe the histomathological manifestations of the hippocampus. Terminal dexynucleotidyl transferase mediated DUTP Nick end labeling(TUNEL) method was used to detect the apoptosis rate of nerve cells. Western blot and quantitative real-time PCR(QRT-PCR) technique were performed to detect protein and mRNA expression levels of cytochrome C(Cyt-C), caspase-3 and caspase-9, respectively.Results Compared with the normal group, rats in the AD group had a longer escape latency from 1 to 5 days after gavage(P<0.05). Compared with the AD group, the escape latency of the intervention group A, the intervention group B, and the intervention group C was all shortened(P<0.05). Additionally, the escape latency of the drug control group was not significantly different from that of the intervention group C(P>0.05). Compared with the normal group, the swimming distance of rats in the AD group was significantly increased(P<0.05). Compared with the AD group, the swimming distance of rats in the intervention group A, the intervention B group and the intervention C group was all re

关 键 词：阿尔茨海默病 黄芪多糖 认知功能 神经细胞 天冬氨酸特异性半胱氨酸蛋白酶-3/9信号通路 

分 类 号：R743[医药卫生—神经病学与精神病学]