急性脑梗死对大鼠树突状细胞和炎症因子水平的影响  

Effects of Acute Cerebral Infarction on the Levels of Dendritic Cells and Inflammatory Factors in Rats

作　　者：钟真真[1] 徐平[1] 王凯[2] 张小波[1] ZHONG Zhen-zhen;XU Ping;WANG Kai(First Peoples Hospital of Changde City,Changde 415000,China)

机构地区：[1]常德市第一人民医院,湖南常德415000 [2]南华大学,湖南衡阳421000

出　　处：《医学临床研究》2021年第10期1515-1518,共4页Journal of Clinical Research

基　　金：湖南省自然科学基金科卫联合项目(2018JJ6002);常德市科技局一般项目(2018S026)。

摘　　要：【目的】研究急性脑梗死对大鼠树突状细胞(Dendritic cells,DCs)和炎症因子水平的影响,【方法】将20只8周龄雌性SD大鼠随机分为实验组和对照组,每组10只。采用线栓法制作大脑中动脉梗死(middle cerebral artery occlusion,MACO)大鼠模型,对照组手术步骤与实验组相同,但不插入线栓。术后7 d行免疫荧光、免疫组化和Elisa检测,观察实验组和对照组DCs、白介素1β(Interleukin-1β,IL-1β)、白介素-6(Interleukin-6,IL-6)和肿瘤坏死因子α(Tumor necrosisfactor-α,TNF-α)的表达水平,【结果】与对照组相比,实验组大鼠缺血侧脑组织CD74和IntegrinaE阳性细胞数较多,差异具有统计学意义(P<0.05);实验组大鼠缺血侧脑组织IL-1β、IL-6和TNF-α的表达量高于对照组,差异具有统计学意义(P<0.05);两组大鼠血清IL-1β、IL-6和TNF-α的含量比较,差异具有统计学意义(P<0.05),【结论】脑梗死诱发DCs及炎症因子的表达;炎症反应是急性脑梗死的病理过程。【Objective】To to study the effects of acute cerebral infarction on the levels of dendritic cells(DCS)and inflammatory factors in rats.【Methods】Twenty 8-week-old female SD rats were randomly divided into experi mental group and control group,with 10 rats in each group.The middle cerebral artery occlusion(MACO)rat model was made by suture method.The operation steps of the control group were the same as those of the experimental group,but the suture was not inserted.Immunofluorescence,immunohistochemistry and ELISA were performed 7 days after operation.Expression levels of DCs and interleukin-1β(IL-1β),Interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in the experimental group and the control group were observed.【Results】Compared with the control group,the quantity of positive cells of CD74 and integrinaE in the ischemic brain tissue of the experimental group was bigger,and the difference was statistically significant(P<0.05);The expression of IL-1β,IL-6 and TNF-α in ischemic brain tissue of rats in experimental group was significantly higher than that in the control group(P<0.05);The difference of the contents of serum IL-1β,IL-6 and TNF-α of the two groups was statistically significant(P<0.05).【Conclusion】Cerebral infarction induces the expression of DCs and inflam matory factors in rats,and the inflammatory response is a pathological process of acute cerebral infarction.

关 键 词：脑梗死 急性病 树突细胞/免疫学 炎症介导素类/血液 疾病模型 动物 大鼠 

分 类 号：R743.33[医药卫生—神经病学与精神病学]