先天性EDNRB基因缺陷对大鼠海马区神经细胞凋亡的影响  

The effection of neural apoptosis in the hippocampal formation of rats modal with congenital EDNRB gene defect

作　　者：谢聃[1] 宋赞民 王霞 张拥波[1] XIE Dan;SONG Zan-min;WANG Xia;ZHANG Yong-bo(Department of Neurology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Brain Development Laboratory,John Curtin School of Medical Research,Australian National University,Canberra ACT2601,Australia;Department of Neurology,The People's Hospital of Wu Hai Inner Mongolia,Wuhai 016000,Inner Mongolia,China)

机构地区：[1]首都医科大学附属北京友谊医院神经内科,100050 [2]澳大利亚国立大学约翰科廷医学研究中心神经研究所,堪培拉ACT2601 [3]内蒙古自治区乌海市人民医院神经内科,016000

出　　处：《中国现代神经疾病杂志》2021年第10期881-886,共6页Chinese Journal of Contemporary Neurology and Neurosurgery

基　　金：内蒙古自治区自然科学基金资助项目(项目编号:2020MS03017)。

摘　　要：目的探讨EDNRB基因缺陷的新生大鼠海马神经细胞凋亡及神经营养因子中脑源性神经营养因子(BDNF)和胶质细胞源性神经营养因子(GDNF)的表达变化。方法制备先天性碱基缺失致EDNRB基因缺陷新生大鼠(出生后2~3 d)模型,并分为野生型(+/+)、杂合子型(+/sl)和纯合子型(sl/sl)共3种基因型(每组各6~8只),TUNEL染色检测海马神经细胞凋亡情况,荧光共聚焦显微镜计数细胞凋亡数目,酶联免疫吸附试验测定海马BDNF和GDNF表达量。结果 3种基因型新生大鼠海马组织凋亡细胞数目差异有统计学意义(F=24.315,P=0.000),sl/sl凋亡细胞数目高于+/+(t=-6.780,P=0.000)和+/sl(t=4.801,P=0.000);海马CA1区凋亡细胞数目最高,CA3区次之,齿状回最低。而3种基因型新生大鼠海马BDNF(F=0.766,P=0.479)和GDNF(F=2.538,P=0.107)表达量差异无统计学意义。结论EDNRB基因缺陷新生大鼠海马CA1区、CA3区神经细胞凋亡增多,而神经营养因子BDNF和GDNF水平无明显变化。Objective The purpose of this study is to investigate apoptosis of hippocampal neurons and the expression of glial cell line-derived neurotrophic factor(GDNF) and brain-derived neurotrophic factor(BDNF) in neonatal pups with EDNRB gene deficiency.Methods The infant rats(2-3 d after birth)with EDNRB gene defect caused by congenital base deletion were divided into wild(+/+),heterozygous(+/sl) and homozygous(sl/sl),6-8 rats were included in each group.Apoptosis of hippocampal nerve cells was detected by TUNEL staining.The number of apoptotosic cells was calculated by confocal fluorescence microscopy,and the levels of GDNF and BDNF in hippocampus was determined by enzyme-linked immunsorbent assay(ELISA).Results The number of apoptotic cells in hippocampus of the three genotypes was significantly different(F=24.315,P=0.000),the number of apoptotic cells in sl/sl was higher than that in+/+(t=-6.780,P=0.000) and+/sl(t=4.801,P=0.000).The number of apoptotic cells was the highest in hippocampal CA1 region,followed by CA3 region,and the lowest in dentate gyrus.However,there was no significant difference in the levels of BDNF(F=0.766,P=0.479) and GDNF(F=2.538,P=0.107) in the hippocampus between different genotypes.Conclusions In rat with EDNRB gene defect,compared with the heterozygote and wild rats,the neural apoptosis were increased in hippocampal CA1,CA3 regions.But the levels of neurotrophic factors BDNF and GDNF were not significantly changed.

关 键 词：受体 内皮素B 基因 海马 细胞凋亡 神经生长因子类 疾病模型 动物 

分 类 号：R741[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]