超高效液相色谱串联质谱法测定甘蔗中3-硝基丙酸的不确定度评定  

Evaluation on Uncertainty of Determination of 3-Nitropropionic Acid in Sugarcane by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

作　　者：罗志荣 庞扬海 黄敏兴 曾秋霞 高裕锋 LUO Zhirong;PANG Yanghai;HUANG Minxing;ZENG Qiuxia;GAO Yufeng(Institute of Biological and Medical Engineering,Guangdong Academy of Sciences,Guangzhou 510316;Research Center for Sugarcane Industry Engineering Technology of Light Industry of China,Guangzhou 510316)

机构地区：[1]广东省科学院生物与医学工程研究所,广东广州510316 [2]中国轻工业甘蔗制糖工程技术研究中心,广东广州510316

出　　处：《甘蔗糖业》2021年第4期54-58,共5页Sugarcane and Canesugar

基　　金：广州市科技计划项目(201806010093)。

摘　　要：分析利用超高效液相色谱串联质谱法(UPLC-MS/MS)测定甘蔗中3-硝基丙酸的不确定度来源,对不确定度的各个分量进行评估和合成。结果表明,影响3-硝基丙酸含量测定的不确定度的主要原因是标准工作曲线配制和重复性测定引入的不确定度分量。当甘蔗中3-硝基丙酸的测定值为152.20μg/kg时,计算得到扩展不确定度为11.62μg/kg,测定结果表示为(152.20±11.62)μg/kg,k=2。The uncertainty of determination of 3-nitropropionic acid in sugarcane by UPLC-MS/MS was evaluated and synthesized. The results showed that the main uncertainty components were preparation of standard working curve and repeatability measurement. When the determination value of 3-nitropropionic acid in sugarcane was 152.20 μg/kg, the expanded uncertainty was calculated to be 11.62 μg/kg, and the determination result was expressed as(152.20±11.62) μg/kg, k=2.

关 键 词：超高效液相色谱串联质谱法 甘蔗 3-硝基丙酸 不确定度 

分 类 号：TS247[轻工技术与工程—制糖工程]