Detailing the ultrastructure’s increase of prion protein in pancreatic adenocarcinoma  

作　　者：Matteo Bianchini Maria Anita Giambelluca Maria Concetta Scavuzzo Gregorio Di Franco Simone Guadagni Matteo Palmeri NiccolòFurbetta Desirée Gianardi Niccola Funel Claudio Ricci Raffaele Gaeta Luca Emanuele Pollina Alfredo Falcone Caterina Vivaldi Giulio Di Candio Francesca Biagioni Carla Letizia Busceti Luca Morelli Francesco Fornai 

机构地区：[1]General Surgery Unit,Department of Translational Research and New Technologies in Medicine and Surgery,University of Pisa,Pisa 56124,Italy [2]Human Anatomy,Department of Translational Research and New Technologies in Medicine and Surgery,University of Pisa,Pisa 56124,Italy [3]Division of Surgical Pathology,Department of Surgical,Medical,Molecular Pathology and Critical Area,University of Pisa,Pisa 56124,Italy [4]Division of Medical Oncology,Department of Translational Research and New Technologies in Medicine and Surgery,University of Pisa,Pisa 56124,Italy [5]IRCCS Neuromed,Istituto Neurologico Mediterraneo,Pozzilli 86077,Italy [6]EndoCAS(Center for Computer Assisted Surgery),University of Pisa,Pisa 56124,Italy

出　　处：《World Journal of Gastroenterology》2021年第42期7324-7339,共16页世界胃肠病学杂志（英文版）

基　　金：by Tizzi Foundation and Arpa Foundation(www.fondazionearpa.it).

摘　　要：BACKGROUND Recent evidences have shown a relationship between prion protein(PrPc)expression and pancreatic ductal adenocarcinoma(PDAC).Indeed,PrPc could be one of the markers explaining the aggressiveness of this tumor.However,studies investigating the specific compartmentalization of increased PrPc expression within PDAC cells are lacking,as well as a correlation between ultrastructural evidence,ultrastructural morphometry of PrPc protein and clinical data.These data,as well as the quantitative stoichiometry of this protein detected by immuno-gold,provide a significant advancement in understanding the biology of disease and the outcome of surgical resection.AIM To analyze quantitative stoichiometry and compartmentalization of PrPc in PDAC cells and to correlate its presence with prognostic data METHODS Between June 2018 and December 2020,samples from pancreatic tissues of 45 patients treated with pancreatic resection for a preoperative suspicion of PDAC at our Institution were collected.When the frozen section excluded a PDAC diagnosis,or the nodules were too small for adequate sampling,patients were ruled out from the present study.Western blotting was used to detect,quantify and compare the expression of PrPc in PDAC and control tissues,such as those of non-affected neighboring pancreatic tissue of the same patient.To quantify the increase of PrPc and to detect the subcellular compartmentalization of PrPc within PDAC cells,immuno-gold stoichiometry within specific cell compartments was analyzed with electron microscopy.Finally,an analysis of quantitative PrPc expression according to prognostic data,such as cancer stage,recurrence of the disease at 12 mo after surgery and recurrence during adjuvant chemotherapy was made.RESULTS The amount of PrPc within specimen from 38 out of 45 patients was determined by semi-quantitative analysis by using Western blotting,which indicates that PrPc increases almost three-fold in tumor pancreatic tissue compared with healthy pancreatic regions[242.41±28.36 optical density(OD)

关 键 词：Pancreatic ductal adenocarcinoma Prion protein Western blotting Electron microscopy Cellular compartmentalization NEUROINVASION 

分 类 号：R735.9[医药卫生—肿瘤]