黄秋葵可溶性和不溶性膳食纤维的提取工艺优化  

Optimization of Extraction Process of Soluble and Insoluble Dietary Fiber from Abelmoschus Esculentus

作　　者：李永平[1,2] 黄春容 康玉妹[1,2] 马慧斐 薛珠政[1,2] 朱海生[1,2] LI Yong-ping;HUANG Chun-rong;KANG Yu-mei;MA Hui-fei;XUE Zhu-zheng;ZHU Hai-sheng(Vegetable Laboratory, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;Crop Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;Luoyuan County Bureau of Agriculture and Rural Affairs, Fuzhou, Fujian 350699, China)

机构地区：[1]福建省农业科学院蔬菜研究室,福建福州350013 [2]福建省农业科学院作物研究所,福建福州350013 [3]福建省罗源县农业农村局,福建福州350699

出　　处：《福建农业科技》2021年第9期1-7,共7页Fujian Agricultural Science and Technology

基　　金：福建省科技计划项目——省属公益类科研院所基本科研专项(2019R1031-4);福建省农业科学院蔬菜遗传育种科技创新团队(CXTD2021038);福建省农业科学院科技创新平台专项(CXPT202001);国家大宗蔬菜产业技术体系福州综合试验站(CARS-23-G-53)。

摘　　要：为优化黄秋葵膳食纤维的提取工艺,以黄秋葵为原料,采用酸解法提取黄秋葵可溶性膳食纤维,以及酶解法提取黄秋葵不溶性膳食纤维,通过单因素试验和正交试验,确定黄秋葵可溶性、不溶性膳食纤维的最佳工艺条件。结果表明:酸解法提取黄秋葵可溶性膳食纤维得率的最主要影响因素为料液比,其次是pH值、提取时间、提取温度,优化最佳提取条件为料液比1∶30(g/mL)、pH值7.0、提取温度100℃、提取时间100 min,此工艺下黄秋葵中可溶性膳食纤维得率可达13.52%;酶法提取黄秋葵不溶性膳食纤维得率影响较大的影响因素是pH和酶解温度,优化最佳提取条件为温度为60℃、pH 5.0、加酶量为0.7%、酶解时间为60 min。此工艺条件下得率可达37.82%。应用优化条件测试6个黄秋葵品种的可溶性膳食纤维得率均在13%以上,不溶性膳食纤维平均得率为36.15%。该提取优化条件的提取工艺得率较高,可为黄秋葵膳食纤维的提取提供参考。In order to optimize the extraction process of dietary fiber from Abelmoschus esculentus,by using Abelmoschus esculentus as the raw material,the soluble dietary fiber was extracted from Abelmoschus esculentus by acid hydrolysis method and the insoluble dietary fiber was extracted from Abelmoschus esculentus by enzymatic hydrolysis method.The optimum technological conditions of the soluble and insoluble dietary fiber in Abelmoschus esculentus were determined by using the single factor experiment and orthogonal experiment.The results showed that the ratio of solid to liquid was the most important factor affecting the yield of soluble dietary fiber extracted from Abelmoschus esculentus by acid hydrolysis method,followed by the value of pH,extraction time and extraction temperature.The optimal extraction conditions were as follows:the solid-liquid ratio 1∶30(g/mL),pH 7.0,extraction temperature 100℃,and extraction time 100 min.And the yield of soluble dietary fiber from Abelmoschus esculentus could reach 13.52%under these conditions.PH and enzymatic hydrolysis temperature were the most influential factors on the yield of insoluble dietary fiber from Abelmoschus esculentus.The optimal extraction conditions were as follows:temperature 60℃,pH 5.0,enzyme dosage 0.7%and enzymatic hydrolysis time 60 min.And the yield could reach 37.82%under these conditions.By using the optimized conditions,the yield of soluble dietary fiber in the six varieties of Abelmoschus esculentus were measured to be above 13%,while the average yield of insoluble dietary fiber was measured to be 36.15%.The extraction yield of dietary fiber from Abelmoschus esculentus under the optimized conditions was higher,which could provide reference for the extraction of dietary fiber from Abelmoschus esculentus.

关 键 词：黄秋葵 膳食纤维 提取 测定 

分 类 号：S649[农业科学—蔬菜学]