Biomimetic Ti-6Al-4V alloy/gelatin methacrylate hybrid scaffold with enhanced osteogenic and angiogenic capabilities for large bone defect restoration  

作　　者：Limin Ma Xiaolan Wang Ye Zhou Xiongfa Ji Shi Cheng Dong Bian Lei Fan Lei Zhou Chengyun Ning Yu Zhang 

机构地区：[1]Department of Orthopedics,Guangdong Provincial People’s Hospital,Guangdong Academy of Medical Sciences,Guangzhou,Guangdong,510080,China [2]Laboratory of Basic Medicine,General Hospital of Southern Theatre Command of the PLA,Guangzhou,510010,China [3]School of Materials Science and Engineering,South China University of Technology,Guangzhou,510641,China

出　　处：《Bioactive Materials》2021年第10期3437-3448,共12页生物活性材料（英文）

基　　金：This work was supported by National Natural Science Foundation of China(31700880,81972126);Natural Science Foundation of Guangdong Province(2020A1515010827);Science and Technology Planning Project of Guangzhou city(201803010106);The China Postdoctoral Science Foundation(2019M652957);Science and Technology Planning Project of Jiangmen City(2019030102490013068);the High-level Hospital Construction Project(KJ012019100)for financial support.

摘　　要：Titanium-based scaffolds are widely used implant materials for bone defect treatment.However,the unmatched biomechanics and poor bioactivities of conventional titanium-based implants usually lead to insufficient bone integration.To tackle these challenges,it is critical to develop novel titanium-based scaffolds that meet the bioadaptive requirements for load-bearing critical bone defects.Herein,inspired by the microstructure and mechanical properties of natural bone tissue,we developed a Ti-6Al-4V alloy(TC4)/gelatin methacrylate(GelMA)hybrid scaffold with dual bionic features(GMPT)for bone defect repair.GMPT is composed of a hard 3D-printed porous TC4 metal scaffold(PT)backbone,which mimics the microstructure and mechanical properties of natural cancellous bone,and a soft GelMA hydrogel matrix infiltrated into the pores of PT that mimics the microenvironment of the extracellular matrix.Ascribed to the unique dual bionic design,the resultant GMPT demonstrates better osteogenic and angiogenic capabilities than PT,as confirmed by the in vitro and rabbit radius bone defect experimental results.Moreover,controlling the concentration of GelMA(10%)in GMPT can further improve the osteogenesis and angiogenesis of GMPT.The fundamental mechanisms were revealed by RNA-Seq analysis,which showed that the concentration of GelMA significantly influenced the expression of osteogenesis-and angiogenesis-related genes via the Pi3K/Akt/mTOR pathway.The results of this work indicate that our dual bionic implant design represents a promising strategy for the restoration of large bone defects.

关 键 词：3D printing porous titanium alloys Gelatin methacrylate Angiogenesis OSTEOGENESIS 

分 类 号：R318.08[医药卫生—生物医学工程]