Covalent functionalization of decellularized tissues accelerates endothelialization  

作　　者：Eleonora Dal Sasso Annj Zamuner Andrea Filippi Filippo Romanato Tiziana Palmosi Luca Vedovelli Dario Gregori Jose Luís Gòmez Ribelles Teresa Russo Antonio Gloria Laura Iop Gino Gerosa Monica Dettin 

机构地区：[1]Department of Cardiac,Thoracic and Vascular Sciences and Venetian Institute of Molecular Medicine,Padua,Italy [2]Department of Industrial Engineering,University of Padua,Padua,Italy [3]LIFELAB Program,Consorzio per la Ricerca Sanitaria,CORIS,Veneto Region,Italy [4]Department of Physics and Astronomy“G.Galilei”,University of Padua,Padua,Italy [5]Fondazione Bruno Kessler,Trento,Italy [6]Institute of Pediatric Research Citt`a della Speranza,Padua,Italy [7]Center for Biomaterials and Tissue Engineering,CBIT,Universitat Polit`ecnica de Val`encia,Val`encia,Spain [8]Biomedical Research Networking Center on Bioengineering,Biomaterials and Nanomedicine(CIBER-BBN),Valencia,Spain [9]Institute of Polymers,Composites and Biomaterials,National Research Council of Italy,Naples,Italy

出　　处：《Bioactive Materials》2021年第11期3851-3864,共14页生物活性材料（英文）

基　　金：Padua Heart Program(CA.RI.PA.RO.Foundation);LIFELAB Program,Consorzio per la Ricerca Sanitaria-CORIS,Veneto Region,Via Giustiniani,2-Padova;JLGR acknowledges financial support from the Spanish State Research Agency(AEI)through the PID2019-106099RB-C41/AEI/10.13039/501100011033 project;CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008-2011,Iniciativa Ingenio 2010,Consolider Program.CIBER Actions are financed by the Instituto de Salud CarlosⅢwith assistance from the European Regional Development Fund.

摘　　要：In the field of tissue regeneration,the lack of a stable endothelial lining may affect the hemocompatibility of both synthetic and biological replacements.These drawbacks might be prevented by specific biomaterial functionalization to induce selective endothelial cell(EC)adhesion.Decellularized bovine pericardia and porcine aortas were selectively functionalized with a REDV tetrapeptide at 10^(-5)M and 10^(-6)M working concentrations.The scaffold-bound peptide was quantified and REDV potential EC adhesion enhancement was evaluated in vitro by static seeding of human umbilical vein ECs.The viable cells and MTS production were statistically higher in functionalized tissues than in control.Scaffold histoarchitecture,geometrical features,and mechanical properties were unaffected by peptide anchoring.The selective immobilization of REDV was effective in accelerating ECs adhesion while promoting proliferation in functionalized decellularized tissues intended for blood-contacting applications.

关 键 词：Decellularized pericardium Decellularized aorta Covalent functionalization ENDOTHELIALIZATION REDV Mechanical analysis 

分 类 号：R318[医药卫生—生物医学工程]