Polylysine-decorated macroporous microcarriers laden with adipose-derived stem cells promote nerve regeneration in vivo  

作　　者：Yi Sun Xiaoqi Chi Haoye Meng Mengjiao Ma Jing Wang Zhaoxuan Feng Qi Quan Guodong Liu Yansen Wang Yajie Xie Yudong Zheng Jiang Peng 

机构地区：[1]School of Materials Science and Engineering,University of Science and Technology Beijing,Beijing,100083,PR China [2]Institute of Orthopedics,Chinese PLA General Hospital,Beijing Key Lab of Regenerative Medicine in Orthopedics,Key Lab of Musculoskeletal Trauma&War Injuries,PLA,No.28 Fuxing Road,Beijing,100853,PR China [3]Qingdao National Laboratory for Marine Science and Technology,Qingdao,266237,PR China [4]Co-innovation Center of Neuroregeneration,Nantong University,Nantong,Jiangsu Province,226007,PR China

出　　处：《Bioactive Materials》2021年第11期3987-3998,共12页生物活性材料（英文）

基　　金：National Natural Science Foundation of China(Grant No.51773018,51973018);Key Research and Development Projects of People’s Liberation Army(BWS17J036).

摘　　要：Cell transplantation is an effective strategy to improve the repair effect of nerve guide conduits(NGCs).However,problems such as low loading efficiency and cell anoikis undermine the outcomes.Microcarriers are efficient 3D cell culture scaffolds,which can also prevent cell anoikis by providing substrate for adhesion during transplantation.Here,we demonstrate for the first time microcarrier-based cell transplantation in peripheral nerve repair.We first prepared macroporous chitosan microcarriers(CSMCs)by the emulsion-phase separation method,and then decorated the CSMCs with polylysine(pl-CSMCs)to improve cell affinity.We then loaded the pl-CSMCs with adipose-derived stem cells(ADSCs)and injected them into electrospun polycaprolactone/chitosan NGCs to repair rat sciatic nerve defects.The ADSCs-laden pl-CSMCs effectively improved nerve regeneration as demonstrated by evaluation of histology,motor function recovery,electrophysiology,and gastrocnemius recovery.With efficient cell transplantation,convenient operation,and the multiple merits of ADSCs,the ADSCs-laden pl-CSMCs hold good potential in peripheral nerve repair.

关 键 词：Chitosan microcarriers POLYLYSINE Adipose-derived stem cells Peripheral nerve repair Cell transplantation 

分 类 号：R318[医药卫生—生物医学工程]