3D bioactive cell-free-scaffolds for in-vitro/in-vivo capture and directed osteoinduction of stem cells for bone tissue regeneration  

作　　者：Mamatali Rahman Xue-Liang Peng Xiao-Hong Zhao Hai-Lun Gong Xiao-Dan Sun Qiong Wu Dai-Xu Wei 

机构地区：[1]Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education,School of Medicine,Department of Life Sciences and Medicine,Northwest University,Xi’an,710069,China [2]MOE Key Laboratory of Bioinformatics,Center for Synthetic and System Biology,Tsinghua University,Beijing,100084,China [3]School of Life Sciences,Tsinghua University,Beijing,100084,China [4]State Key Laboratory of New Ceramics and Fine Processing,School of Materials Science and Engineering,Tsinghua University,Beijing,100084,China [5]Key Laboratory of Advanced Materials of Ministry of Education of China,School of Materials Science and Engineering,Tsinghua University,Beijing,100084,China

出　　处：《Bioactive Materials》2021年第11期4083-4095,共13页生物活性材料（英文）

基　　金：Grants from National Natural Science Foundation of China(Grant Nos.31900950,31670991 and 52072210);National Key Research and Development Project of China(Grant No.2018YFA0900100).

摘　　要：Hydrophilic bone morphogenetic protein 2(BMP2)is easily degraded and difficult to load onto hydrophobic carrier materials,which limits the application of polyester materials in bone tissue engineering.Based on soybean-lecithin as an adjuvant biosurfactant,we designed a novel cell-free-scaffold of polymer of poly(ε-caprolactone)and poly(lactide-co-glycolide)-co-polyetherimide with abundant entrapped and continuously released BMP2 for in vivo stem cell-capture and in situ osteogenic induction,avoiding the use of exogenous cells.The optimized bioactive osteo-polyester scaffold(BOPSC),i.e.SBMP-10SC,had a high BMP2 entrapment efficiency of 95.35%.Due to its higher porosity of 83.42%,higher water uptake ratio of 850%,and sustained BMP2 release with polymer degradation,BOPSCs were demonstrated to support excellent in vitro capture,proliferation,migration and osteogenic differentiation of mouse adipose derived mesenchymal stem cells(mADSCs),and performed much better than traditional BMP-10SCs with unmodified BMP2 and single polyester scaffolds(10SCs).Furthermore,in vivo capture and migration of stem cells and differentiation into osteoblasts was observed in mice implanted with BOPSCs without exogenous cells,which enabled allogeneic bone formation with a high bone mineral density and ratios of new bone volume to existing tissue volume after 6 months.The BOPSC is an advanced 3D cell-free platform with sustained BMP2 supply for in situ stem cell capture and osteoinduction in bone tissue engineering with potential for clinical translation.

关 键 词：Soybean lecithin Bioactive material Cell-free scaffold BMP2 Cell-capture OSSIFICATION 

分 类 号：R318[医药卫生—生物医学工程] R68[医药卫生—基础医学]