β-1,4-半乳糖基转移酶Ⅴ对脂多糖模型中iNOS、TNFR表达的影响  

Effects ofβ-1,4-galactosyltransferaseⅤon expression of iNOS and TNFR in lipopolysaccharide model

作　　者：王小瑜 高书美 周巧 金会 惠美琦 严美娟[1] 沈洪妹[1] WANG Xiaoyu;GAO Shumei;ZHOU Qiao;JIN Hui;HUI Meiqi;YAN Meijuan;SHEN Hongmei(Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,Co-innovation Center of Neuroregeneration,Nantong University,Nantong 226001)

机构地区：[1]南通大学教育部/江苏省神经再生重点实验室/神经再生协同创新中心,南通226001

出　　处：《南通大学学报（医学版）》2021年第5期399-402,共4页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(31370803);南通市科技计划重点项目(MS22021009)。

摘　　要：目的:探索β-1,4-半乳糖基转移酶Ⅴ(β-1,4-galactosyltransferaseⅤ,β-1,4-GalTⅤ)对腹腔注射脂多糖(lipopolysaccharide,LPS)引起脑皮层诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)、肿瘤坏死因子受体(tumor necrosis factor receptor,TNFR)变化的调控作用。方法:腹腔注射LPS(0.5 mg/kg)建立炎症模型,采用组织免疫荧光染色观察iNOS与β-1,4-GalTⅤ在脑组织中的表达;采用实时荧光定量聚合酶链式反应(quantitative real-time polymerase chain reaction,RT-PCR)检测脑组织中iNOS的mRNA表达;采用免疫印迹检测脑组织中TNFR1、TNFR2蛋白表达;采用脑皮层定点注射β-1,4-GalTⅤ的沉默或者过表达的慢病毒颗粒,观察β-1,4-GalTⅤ对脂多糖引起iNOS、TNFR变化的影响。结果:在LPS模型中,β-1,4-GalTⅤ和iNOS在脑组织中共定位;LPS刺激引起脑组织中iNOS、TNFR1表达增加,β-1,4-GalTⅤ沉默消减LPS诱导引起的iNOS、TNFR1水平增加;LPS刺激和β-1,4-GalTⅤ表达变化不影响脑组织中TNFR2表达。结论:β-1,4-GalTⅤ对脂多糖引起iNOS、TNFR1变化具有特异的调控作用,但不影响TNFR2的表达。Objective:To explore the regulative effect ofβ-1,4-galactosyltransferaseⅤ(β-1,4-GalTⅤ)on inducible nitric oxide synthase(iNOS)and tumor necrosis factor receptor(TNFR)of cerebral cortex in lipopolysaccharide(LPS)induced inflammatory model by intraperitoneal injection.Methods:Inflammatory model was established by intraperitoneal injection of LPS(0.5 mg/kg),and iNOS andβ-1,4-GalTⅤwere observed in cerebral cortex by tissue immunofluorescence staining.The mRNA expression of iNOS in cerebral cortex was detected by quantitative real-time polymerase chain reaction(RT-PCR),and the protein expression of TNFR1 and TNFR2 was tested by immunoblotting.The effect ofβ-1,4-GalTⅤon expression of iNOS and TNFR in LPS induced inflammatory model was tested by injection withβ-1,4-GalTⅤRNAi orβ-1,4-GalTⅤoverexpression lentivirus particles in cerebral cortex.Results:The co-localization ofβ-1,4-GalTⅤand iNOS was observed in cerebral cortex in LPS rat model.LPS stimulation induced the increase in iNOS and TNFR1,which was eliminated byβ-1,4-GalTⅤRNAi,but expression of TNFR2 was not affected by LPS stimulation andβ-1,4-GalTⅤ.Conclusion:β-1,4-GalTⅤhas a specific regulative effect on expression of iNOS and TNFR1 in LPS model,but it has no effect on TNFR2.

关 键 词：β-1 4-半乳糖基转移酶Ⅴ 脂多糖 诱导型一氧化酶 肿瘤坏死因子受体 大鼠 

分 类 号：R364.5[医药卫生—病理学]