Effects of different aperture-sized type I collagen/silk fibroin scaffolds on the proliferation and differentiation of human dental pulp cells  

作　　者：Shihui Jiang Zhaoxia Yu Lanrui Zhang Guanhua Wang Xiaohua Dai Xiaoli Lian Yan Yan Linpu Zhang Yue Wang Ruixin Li Huiru Zou 

机构地区：[1]Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction,Tianjin Stomatological Hospital,The Affiliated Stomatological Hospital of Nankai University,No.75 Dagu Road,Heping District,Tianjin 300041,China [2]School of Medicine,Nankai University,No.94 Weijin Road,Nankai District,Tianjin 300071,China [3]State Key Laboratory of Medicinal Chemical Biology,Nankai University,No.94 Weijin Road,Nankai District,Tianjin 300071,China

出　　处：《Regenerative Biomaterials》2021年第4期42-50,共9页再生生物材料（英文版）

基　　金：This work was supported by the Natural Science Foundation of Tianjin City of China(grant number 18JCYBJC27000);the National Natural Science Foundation of China(grant number 11972198);the State Key Laboratory of Medicinal Chemical Biology(grant number 2018012);the Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction(grant number KFKT2017008);the Tianjin Health and Family Planning Commission of the People’s Republic of China(grant numbers ZD20016,2014KY24 and 2015KY23).

摘　　要：This study aimed at evaluate the effects of different aperture-sized type I collagen/silk fibroin(CSF)scaffolds on the proliferation and differentiation of human dental pulp cells(HDPCs).The CSF scaffolds were designed with 3D mapping software Solidworks.Three different aperture-sized scaffolds(CSF1-CSF3)were prepared by low-temperature deposition 3D printing technology.The morphology was observed by scanning electron microscope(SEM)and optical coherence tomography.The porosity,hydrophilicity and mechanical capacity of the scaffold were detected,respectively.HDPCs(third passage,1105 cells)were seeded into each scaffold and investigated by SEM,CCK-8,alkaline phosphatase(ALP)activity and HE staining.The CSF scaffolds had porous structures with macropores and micropores.The macropore size of CSF1 to CSF3 was 421627 lm,579636 lm and 707643 lm,respectively.The porosity was 69.862.2%,80.162.8%and 86.563.3%,respectively.All these scaffolds enhanced the adhesion and proliferation of HDPCs.The ALP activity in the CSF1 group was higher than that in the CSF3 groups(P<0.01).HE staining showed HDPCs grew in multilayer within the scaffolds.CSF scaffolds significantly improved the adhesion and ALP activity of HDPCs.CSF scaffolds were promising candidates in dentine-pulp complex regeneration.

关 键 词：tissue engineering dentine-pulp complex regeneration COLLAGEN silk fibroin odontogenic differentiation 

分 类 号：R78[医药卫生—口腔医学] R318[医药卫生—临床医学]