MMR缺失型子宫内膜癌E-cadherin、N-cadherin表达与临床病理特征的相关性分析  

Correlation Analysis between Expression of E-Cadherin and N-Cadherin and Clinicopathological Characters in Endometrial Cancer with MMR Protein Deficiency

作　　者：潘婷 马静文[2] 黄娅妮 袁丽薇 赵世云 元娟娟 胡明月 刘丹[2,3] PAN Ting;MA Jingwen;HUANG Yani(Ningxia Medical University,Yinchuan Ningxia 750004,China;Department Gynecology in General Hospital Affiliate to Ningxia Medical University,Yinchuan Ningxia 750004,China)

机构地区：[1]宁夏医科大学,宁夏银川750004 [2]宁夏医科大学总医院妇科,宁夏银川750004 [3]宁夏医科大学生育力保持教育部重点实验室,宁夏银川750004 [4]滨州市中心医院

出　　处：《实用妇产科杂志》2021年第10期772-776,共5页Journal of Practical Obstetrics and Gynecology

摘　　要：目的:分析错配修复(MMR)蛋白缺失型子宫内膜癌患者临床病理特征与上皮型钙黏蛋白(E-cadherin)、神经型钙黏蛋白(N-cadherin)表达之间的相关性。方法:选择2016年1月至2018年6月在宁夏医科大学总医院手术治疗,术后病理确诊为子宫内膜癌伴MMR缺失即MMR(-)的子宫内膜癌患者40例,另选择同期MMR表达正常即MMR(+)的子宫内膜癌患者40例为对照组,E-cadherin、N-cadherin单克隆抗体行石蜡组织切片免疫组化染色,对比性分析其表达与临床病理特征的相关性。结果:MMR(-)型与MMR(+)型子宫内膜癌对比,在年龄、子宫肌层浸润深度、肿瘤累及子宫下段、阴道断端及宫旁软组织浸润、淋巴结转移中,差异无统计学意义(P>0.05),在病理类型、国际妇产科联盟(Federation of Obstetrics and Gynaecology,FIGO)分期、组织学分化程度和淋巴脉管浸润(lymph-vascular space invasion,LVSI)中,差异有统计学意义(P<0.05);MMR(-)型子宫内膜癌组织中,E-cadherin阳性率、总染色强度、阳性细胞数均低于MMR(+)型,而N-cadherin表达呈相反趋势,差异有统计学意义(P<0.01);MMR(-)型子宫内膜癌组织中,E-cadherin、N-cadherin表达呈负相关(r=-0.678,P<0.01)。结论:MMR(-)型子宫内膜癌与MMR(+)型子宫内膜癌组织E-cadherin/N-cadherin表达趋势不同,具有不同的上皮间质转化水平,可能导致临床病理特征差异的形成。Objective:To analyze the correlation between the clinicopathological features and the expression of E-cadherin and N-cadherin in patients with MMR protein-deficient endometrial carcinoma.Methods:40 cases of postoperative pathological confirmed endometrial cancer patients with MMR(-)were selected in our hospital from January 2016 to June 2018,40 cases with endometrial carcinoma with normal MMR expression,in addition,MMR(+),were selected as the control group.Immunohistochemical staining of paraffin-embedded specimens sections was performed with E-Cadherin and N-Cadherin monoclonal antibodies.The correlation between the expression of E-Cadherin,N-Cadherin and clinicopathological parameters were comparatively analyzed.Results:There was no significant difference between MMR(-)and MMR(+)endometrial carcinoma in age,depth of infiltration of myometrium,lower site tumor infiltration in uterine cavity,vaginal and paracervical invasive and lymph node metastasis(P>0.05),and there were significant differences in pathological type,FIGO stage,histological differentiation and LVSI(P<0.05).The positive rate of E-Cadherin,total staining intensity and number of positive cells in MMR(-)endometrial cancer tissue were lower than those in MMR(+)type,but the expression of N-cadherin showed opposite trend.The differences were statistically significant(P<0.01).The expression of E-cadherin and N-cadherin was negatively correlated in MMR(-)EC(r=-0.678,P<0.01).Conclusions:The expression trend of E-cadherin/N-cadherin in MMR(-)EC and MMR(+)EC is different,with different levels of epithelial-mesenchymal transformation,which may lead to the differences of clinicopathological characteristics.

关 键 词：错配修复蛋白 子宫内膜癌 上皮型钙黏蛋白 神经型钙黏蛋白 

分 类 号：R737.33[医药卫生—肿瘤]