SMC4基因在脑胶质瘤中的表达及其临床意义  被引量:1

Structural maintenance of chromosome 4 gene expression in glioma and its clinical significance

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作  者:郭瑶 陈鹏 黄锐 肖爵贤 程祖珏[1] Guo Yao;Chen Peng;Huang Rui;Xiao Juexian;Cheng Zujue(Department of Neurosurgery,Second Affiliated Hospital of Nanchang University,Nanchang 330000,China;Department of Neurosurgery,Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan 528401,China)

机构地区:[1]南昌大学第二附属医院神经外科,南昌330000 [2]中山市中医院神经外科,528401

出  处:《中华神经医学杂志》2021年第10期981-988,共8页Chinese Journal of Neuromedicine

基  金:江西省卫生健康委科技计划项目(20204345);江西省卫生健康委星火推广计划(20208031)。

摘  要:目的分析染色体结构维持蛋白4(SMC4)基因在脑胶质瘤中的表达情况及其临床意义。方法(1)下载中国脑胶质瘤基因组图谱计划(CGGA)数据库中749例胶质瘤组织的SMC4 mRNA表达数据及其患者的临床资料。采用单因素和多因素Cox回归分析明确胶质瘤患者预后不良的独立影响因素。绘制并比较SMC4 mRNA高表达组、SMC4 mRNA低表达组患者的生存曲线。采用受试者工作特征(ROC)曲线分析SMC4 mRNA对患者1、3、5年生存率的预测效能。(2)使用基因表达谱数据动态分析(GEPIA)评价癌症基因组图谱(TCGA)数据库中胶质瘤组织的SMC4 mRNA表达水平与其患者总生存率的关系。通过人类蛋白质图谱(HPA)在线数据库测定高级别胶质瘤(胶质母细胞瘤)、低级别胶质瘤中SMC4蛋白的表达。(3)基因组富集分析比较SMC4 mRNA高表达组、SMC4 mRNA低表达组胶质瘤间基因本体(GO)和京都基因与基因组百科全书(KEGG)通路的差异。采用基因共表达分析与SMC4 mRNA存在相关关系的基因。结果(1)多因素Cox回归分析显示SMC4 mRNA(HR=1.314,95%CI:1.209~1.428,P=0.000)、原发-复发-继发(PRS)类型、WHO分级、年龄、化疗、异柠檬酸脱氢酶(IDH)突变和染色体1p/19q缺失是胶质瘤患者预后不良的独立影响因素。生存分析显示SMC4 mRNA低表达组患者的总生存率高于SMC4 mRNA高表达组,差异有统计学意义(P<0.05)。ROC曲线分析显示,SMC4 mRNA预测患者1年、3年和5年生存率的曲线下面积(AUC)分别为0.712、0.784、0.791。(2)低级别、高级别胶质瘤中SMC4 mRNA表达水平均高于正常对照脑组织,差异均有统计学意义(P<0.05)。低级别胶质瘤中SMC4 mRNA低表达组患者的总生存率高于SMC4 mRNA高表达组,差异有统计学意义(P<0.05)。高级别胶质瘤中SMC4 mRNA低表达组与SMC4 mRNA高表达组患者的总生存率差异无统计学意义(P>0.05)。低级别胶质瘤组织中SMC4蛋白表达显著低于高级别胶质瘤。(3)基因�Objective To analyze the expression of structural maintenance of chromosome 4(SMC4)gene in glioma and its clinical significance.Methods(1)The SMC4 mRNA expression data of 749 glioma tissues and clinical data of these glioma patients were downloaded from Chinese Glioma genome Atlas(CGGA)database.Univariate Cox analysis and multivariate Cox analysis were used to analyze the independent influencing factors for poor prognosis in patients with glioma.Patients were divided into high SMC4 mRNA expression group and low SMC4 mRNA expression group;Kaplan-Meier method was used to draw the survival curve of these patients.Receiver operating characteristic(ROC)curve was used to analyze the predictive efficacy of SMC4 mRNA in 1-,3-and 5-year survival of these patients.(2)The relationships between SMC4 mRNA expression levels in glioma tissues from cancer Genome Atlas(TCGA)database and overall survival of these glioma patients were analyzed by gene expression profiling interactive analysis(GEPIA).The SMC4 protein expressions in high-grade glioma(glioblastoma)and low-grade glioma were determined by human protein atlas(HPA)online database.(3)Genomic enrichment analysis was used to analyze and compare the differences of gene body(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway in gliomas between SMC4 mRNA high expression group and SMC4 mRNA low expression group.Co-expression analysis was performed to analyze the genes related to SMC4 mRNA.Results(1)Multivariate Cox analysis showed that SMC4 mRNA(HR=1.314,95%CI:1.209-1.428,P=0.000),glioma primary-recurrent-secondary(PRS)type,glioma WHO grading,age,chemotherapy,isocitrate dehydrogenase(IDH)mutation and 1P/19q deletion were independent influencing factors for poor prognosis of glioma patients.Survival analysis showed that the overall survival of patients with low SMC4 mRNA expression was significantly higher than that of patients with high SMC4 mRNA expression(P<0.05).The area under the curve(AUC)of ROC was 0.712,0.784 and 0.791,respectively,in predicting 1-year survival r

关 键 词:神经胶质瘤 染色体结构维持蛋白4 生物信息学分析 

分 类 号:R739.41[医药卫生—肿瘤]

 

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