AGTR1及IL-6基因多态性与动脉瘤性蛛网膜下腔出血的关系研究  

Relations between angiotensin II receptor 1 and interleukin-6 gene polymorphisms and aneurysmal subarachnoid hemorrhage

作　　者：王栋[1] 赵海洋 汪仲伟 Wang Dong;Zhao Haiyang;Wang Zhongwei(Department of Neurosurgery,Affiliated Nanyang Second People's Hospital,Xinxiang Medical University,Nanyang 473012,China)

机构地区：[1]新乡医学院附属南阳市第二人民医院神经外科,473012

出　　处：《中华神经医学杂志》2021年第10期1016-1021,共6页Chinese Journal of Neuromedicine

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20191469)。

摘　　要：目的探讨血管紧张素Ⅱ受体1(AGTR1)及白细胞介素-6(IL-6)基因多态性与动脉瘤性蛛网膜下腔出血的关系。方法选取新乡医学院附属南阳市第二人民医院神经外科自2018年5月至2020年5月收治的142例动脉瘤性蛛网膜下腔出血患者和284例普通体检者分别作为观察组、对照组,采集2组受试者空腹外周静脉血,进行基因组DNA提取和PCR扩增,使用限制性内切酶对扩增产物进行酶切反应,比较2组受试者、不同临床特征的动脉瘤性蛛网膜下腔出血患者AGTR1基因A1166C位点和IL-6基因C-572G位点多态性的差异。结果(1)与对照组比较,观察组AGTR1基因A1166C位点的AA基因型频率增高,AC+CC基因型频率降低,差异均有统计学意义(P<0.05);与对照组比较,观察组AGTR1基因A1166C位点A等位基因频率增高,C等位基因频率降低,差异均有统计学意义(P<0.05)。与对照组比较,观察组IL-6基因C-572G位点的CC基因型频率降低,CG+GG基因型频率增高,差异均有统计学意义(P<0.05);与对照组比较,观察组IL-6基因C-572G位点C等位基因频率降低,G等位基因频率增高,差异均有统计学意义(P<0.05)。(2)与未合并糖尿病的动脉瘤性蛛网膜下腔出血患者比较,合并糖尿病的动脉瘤性蛛网膜下腔出血患者AGTR1基因A1166C位点的AA基因型频率增高,AC+CC基因型频率降低,差异均有统计学意义(P<0.05);多发、合并糖尿病的动脉瘤性蛛网膜下腔出血患者的IL-6基因C-572G位点的CC基因型频率分别低于单发、未合并糖尿病的动脉瘤性蛛网膜下腔出血患者,CG+GG基因型频率分别高于单发、未合并糖尿病的动脉瘤性蛛网膜下腔出血患者,差异均有统计学意义(P<0.05)。结论携带AGTR1基因A1166C位点的AA基因型者易患动脉瘤性蛛网膜下腔出血,且合并糖尿病者患病几率更高。携带IL-6基因C-572G位点的CG+GG基因者易患动脉瘤性蛛网膜下腔出血,且合并糖尿病、多发动脉瘤者患病几�Objective To investigate the relations between gene polymorphisms of angiotensin II receptor 1(AGTR1)and interleukin-6(IL-6)and aneurysmal subarachnoid hemorrhage(SAH).Methods One hundred and forty-two patients with aneurysmal subarachnoid hemorrhage admitted to our hospital from May 2018 to May 2020 were selected as an observation group,and 284 healthy subjects underwent physical examination at the same period were selected as a control group.Fasting peripheral venous blood was collected for genomic DNA extraction and PCR amplification.Restriction endonuclease was used to perform enzyme digestion reaction on the amplified products,and the differences of AGTR1 gene polymorphism at A1166C locus and IL-6 gene polymorphism at C-572G locus were compared between the two groups and among the SAH patients with different clinical features.Results(1)The frequency of AA genotype in AGTR1 gene at A1166C locus in the observation group was significantly higher than that in the control group,while that of AC+CC genotype in the observation group was significantly lower than that in the control group(P<0.05);the observation group had significantly higher frequency of A allele and statistically lower frequency of C allele than the control group(P<0.05).The CC genotype frequency in IL-6 gene at C-572G locus in the observation group was significantly lower than that in the control group and the CG+GG genotype frequency were significantly higher than that in the control group(P<0.05);the observation group had significantly lower C allele frequency and significantly higher G allele frequency than the control group(P<0.05).(2)AA genotype frequency in AGTR1 gene at A1166C locus in SAH patients with diabetes mellitus was significantly higher than that in SAH patients without diabetes mellitus,while AC and CC genotype frequency was significantly lower than that in SAH patients without diabetes mellitus(P<0.05).The frequency of CC genotype in IL-6 gene at C-572G locus in SAH patients with multiple aneurysms or diabetes mellitus was signif

关 键 词：颅内动脉瘤 蛛网膜下腔出血 血管紧张素Ⅱ受体1 白细胞介素-6 基因多态性 

分 类 号：R743.35[医药卫生—神经病学与精神病学]