Ionizing Radiation-Induced RPL23a Reduction Regulates Apoptosis via RPL11-MDM2-p53 Pathway in Mouse Spermatogonia  

作　　者：HE Yu Xuan GUO Yi Xiao ZHANG Yong HU Jun Jie DONG Wei Tao DU Xiang Hong ZHAO Xing Xu 

机构地区：[1]College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,Gansu,China [2]Department of Radiation Oncology,Gansu Provincial Hospital,Lanzhou 730000,Gansu,China

出　　处：《Biomedical and Environmental Sciences》2021年第10期789-802,共14页生物医学与环境科学（英文版）

基　　金：the National Natural Science Foundation of China[31902339];the Foundation for Youth Doctor of Gansu Province[2021QB-026];the Youth Talent Program of“Fuxi”[Gaufx-03Y02];the Scientific Research Start-up Funds for Openly-Recruited Doctors of Gansu Agricultural University[2017RCZX-13];the Special Funds for Discipline Construction of Gansu Agricultural University[GAU-XKJS-2018-067]。

摘　　要：Objective The expression patterns of ribosomal large subunit protein 23 a(RPL23 a)in mouse testes and GC-1 cells were analyzed to investigate the potential relationship between RPL23 a expression and spermatogonia apoptosis upon exposure to X-ray.Methods Male mice and GC-1 cells were irradiated with X-ray,terminal dUTP nick end-labelling(TUNEL)was performed to detect apoptotic spermatogonia in vivo.Apoptotic rate and cell cycle phase of GC-1 cells were analyzed with flow cytometry.Protein interactions were detected by Immunoprecipitation and protein localization as studied by immunofluorescence.Immunoblotting and real-time PCR were applied to analyze to protein and gene expression.Results Ionizing radiation(IR)increased spermatogonia apoptosis,the expression of RPL11,MDM2 and p53,and decreased RPL23 a expression in mice spermatogonia in vivo and in vitro.RPL23 a knockdown weakened the interaction between RPL23 a and RPL11,leading to p53 accumulation.Moreover,knockdown and IR decreased RPL23 a that induces spermatogonia apoptosis via RPL23 a-RPL11-MDM2-p53 pathway in GC-1 cells.Conclusion These results suggested that IR reduced RPL23 a expression,leading to weakened the RPL23 a-RPL11 interactions,which may have activated p53,resulting in spermatogonia apoptosis.These results provide insights into environmental and clinical risks of radiotherapy following exposure to IR in male fertility.The graphical abstract was available in the web of www.besjournal.com.

关 键 词：Ionizing radiation TESTIS Spermatogonia apoptosis 

分 类 号：R818.02[医药卫生—放射医学]