miR-377和FNBP1 mRNA在弥漫大B细胞淋巴瘤中的表达及临床意义  

The expression and clinical significance of miR-377 and FNBP1 mRNA in diffuse large B-cell lymphoma

作　　者：王欣如 张丽[1] 肖太武[1] 李强[1] 杜卉 高磊[1] Wang Xinru;Zhang Li;Xiao Taiwu;Li Qiang;Du Hui;Gao Lei(Department of Hematology, Liaocheng People's Hospital, Shandong Province, Liaocheng 252000,China)

机构地区：[1]山东省聊城市人民医院血液科,252000

出　　处：《疑难病杂志》2021年第11期1095-1099,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：山东省医药卫生科技发展计划项目(2019WS0113)。

摘　　要：目的检测弥漫大B细胞淋巴瘤组织中微小RNA-377(miR-377)和形成素结合蛋白1(FNBP1)信使核糖核酸(mRNA)表达及其在发病过程中的作用及临床意义。方法选取2015年2月—2018年3月聊城市人民医院血液科确诊的弥漫大B细胞淋巴瘤患者130例为观察组,同期选取在医院就诊的淋巴结反应性增生患者55例为对照组。采用实时荧光定量PCR法检测弥漫大B细胞淋巴瘤组织和淋巴结反应性增生组织中miR-377、FNBP1 mRNA表达水平;分析二者之间的相关性及与患者临床病理参数之间的关系;使用Kaplan-Meier曲线分析弥漫大B细胞淋巴瘤患者3年总生存率;Cox回归模型分析影响弥漫大B细胞淋巴瘤患者预后的因素。结果观察组miR-377表达水平显著低于对照组,FNBP1 mRNA表达水平显著高于对照组(t/P=7.366/0.000、14.162/0.000)。miR-377低表达亚组临床Ⅲ~Ⅳ期、病理非生发中心型、有全身症状患者比例明显高于miR-377高表达亚组(χ^(2)/P=6.660/0.010、5.004/0.025、6.635/0.010),FNBP1 mRNA低表达亚组临床Ⅲ~Ⅳ期、病理非生发中心型、有全身症状患者比例明显低于FNBP1 mRNA高表达亚组(χ^(2)/P=9.076/0.003、18.593/0.000、10.916/0.001)。miR-377与FNBP1 mRNA表达水平呈负相关(r/P=-0.355/0.000)。治疗后3年总生存率比较,miR-377高表达亚组高于miR-377低表达亚组,FNBP1 mRNA高表达亚组低于FNBP1 mRNA低表达亚组(χ^(2)/P=4.964/0.026、4.550/0.033)。miR-377低表达、FNBP1 mRNA高表达、临床分期Ⅲ~Ⅳ期是影响弥漫大B细胞淋巴瘤患者预后的独立危险因素[OR(95%CI)=2.001(1.412~2.836)、1.855(1.278~2.692)、1.594(1.136~2.237)]。结论弥漫大B细胞淋巴瘤组织中miR-377表达明显降低,FNBP1 mRNA表达明显升高,且其表达与患者预后密切相关,miR-377和FNBP1 mRNA可作为弥漫大B细胞淋巴瘤病情评估及不良预后预测的潜在生物学指标。Objective To detect the expression of microRNA377(miR-377)and morphogen-binding protein 1(FNBP1)messenger ribonucleic acid(mRNA)in diffuse large B-cell lymphoma tissues and their role in the pathogenesis and clinical significance.Methods A total of 130 patients with diffuse large B-cell lymphoma diagnosed in the Department of Hematology,Liaocheng People's Hospital from February 2015 to March 2018 were selected as the observation group,and 55 patients with reactive lymph node hyperplasia who were in the hospital during the same period were selected as the control group.Real-time fluorescent quantitative PCR was used to detect the expression levels of miR-377 and FNBP1 mRNA in diffuse large B-cell lymphoma tissue and lymph node reactive hyperplasia tissue.Analyze the correlation between the two and the relationship with the patient's clinicopathological parameters;use Kaplan Meier curve to analyze the 3-year overall survival rate of patients with diffuse large B-cell lymphoma.Cox regression model analyzes the factors affecting the prognosis of patients with diffuse large B-cell lymphoma.Results The expression level of miR-377 in the observation group was significantly lower than that of the control group,and the expression level of FNBP1 mRNA was significantly higher than that of the control group(t/P=7.366/0.000,14.162/0.000).The proportion of patients with clinical stage III-IV,pathological non-germinal center,and systemic symptoms in the subgroup of miR-377 low expression was significantly higher than that of the subgroup of miR-377 high expression(χ^(2)/P=6.660/0.010,5.004/0.025,6.635/0.010),the proportion of patients with clinical stage III-IV,pathological non-germinal center,and systemic symptoms in the subgroup with low FNBP1 mRNA expression was significantly lower than that of the subgroup with high FNBP1 mRNA expression(χ^(2)/P=9.076/0.003,18.593/0.000,10.916/0.001).There was a negative correlation between miR-377 and FNBP1 mRNA expression level(r/P=-0.355/0.000).Compared with the 3-year overall survival

关 键 词：弥漫大B细胞淋巴瘤 微小RNA-377 形成素结合蛋白1 预后 相关性 

分 类 号：R733.7[医药卫生—肿瘤]