基于16S rRNA检测分析宫颈癌患者放射治疗前后肠道菌群变化特征  

Analysis of the Characteristics of the Changes in Intestinal Flora of Cervical Cancer Patients Before and After Radiotherapy Based on 16S rRNA Detection

作　　者：陈雅云[1] 孙育新[2] 李凯新[1] 高炳宗 陈晓婷[1] 毛润芝 蔡文杰[1] CHEN Ya-yun;SUN Yu-xin;LI Kai-xin;GAO Bing-zong;CHEN Xiao-ting;MAO Run-zhi;CAI Wen-jie(Quanzhou First Hospital Affiliated to Fujian Medical University,Fujian Quanzhou 362000;The Second Affiliated Hospital of Fujian Medical University,Fujian Quanzhou 362000)

机构地区：[1]福建医科大学附属泉州第一医院,福建泉州362000 [2]福建医科大学附属第二医院,福建泉州362000

出　　处：《深圳中西医结合杂志》2021年第17期8-11,F0003,共5页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：泉州市科技计划项目资助课题(2018N055S)。

摘　　要：目的:探究电离辐射对宫颈癌患者肠道菌群的影响特点及菌群变化与放射性肠炎发生的相关性。方法:选取福建医科大学附属泉州第一医院2019年1月至2020年1月收治的30例宫颈癌患者,在其调强放射治疗的不同时间点收集其粪便样本,分析肠道微生态的生物标志物,用MoBio PowerSoil试剂盒提取肠道菌群DNA,用MiniSeq对肠道菌群16S rRNA的V4区进行测序及生物信息学分析。差异度采用Wilcoxon秩和检验等分析。结果:宫颈癌患者放射治疗后肠道微生物群的α多样性比放疗前高,同时放疗前后微生物群落组成(即β多样性)存在一定差异,但差异均无统计学意义(P>0.05);但是在宫颈癌患者放疗后微生物组成中普雷沃氏菌和链球菌较放疗前有显著变化(P<0.05)。结论:宫颈癌患者放疗前后微生物成分存在差异,但有待大规模的前瞻性队列研究证实。Objective To explore the characteristics of the effects of radiation on the intestinal flora and the correlation between changes in the flora and the occurrence of radiation enteritis.Methods Thirty patients with cervical cancer admitted to Quanzhou First Hospital Affiliated to Fujian Medical University from January 2019 to January 2020 were selected.Fecal samples were collected at different time points during IMRT radiotherapy to analyze biomarkers of intestinal microecology.The DNA of intestinal flora was extracted with the MoBio PowerSoil kit.The V4 region of 16S rRNA of intestinal flora was sequenced and analyzed by MiniSeq.Wilcoxon rank sum test was used for difference degree.Results The alpha diversity of the intestinal flora after radiotherapy in patients with cervical cancer was higher than that before radiotherapy.At the same time,there were certain differences in the composition of the microbial community(ie,beta diversity)before and after radiotherapy,but the differences were not statistically significant(P>0.05);After radiotherapy for patients with cervical cancer,we observed significant changes in the microbial composition of Prevotella and Streptococcus compared to before radiotherapy(P<0.05).Conclusions There are differences in the microbial composition of cervical cancer patients before and after radiotherapy,but this needs to be confirmed by a large-scale prospective cohort study.

关 键 词：宫颈癌 肠道菌群 放射性直肠炎 16S核糖体RNA基因测序 

分 类 号：R737.33[医药卫生—肿瘤] R516.1[医药卫生—临床医学]