亚麻荠籽多糖提取工艺的响应面优化及其体外抗氧化活性  

Optimization of extraction process of polysaccharides from Camelina sativa(L.)Crantz seeds cake with response surface methodology and its antioxidant activity

作　　者：朱杰 张华南[1,2,3] 范毅 于立芹 李晓[1,2,3] 魏悦 张海希[4] 李建[1,2,3] 全彦涛 崔炜[1,2,3] 李自红[1,2,3] ZHU Jie;ZHANG Huanan;FAN Yi;YU Liqin;LI Xiao;WEI Yue;ZHANG Haixi;LI Jian;QUAN Yantao;CUI Wei;LI Zihong(Henan Napu Biotechnology Co.,Ltd.,Zhengzhou 450002,China;Biotechnology Developing Center of Henan Academy of Sciences,Zhengzhou 450002,China;Henan Academy of Sciences,Zhengzhou 450002,China;Anyang Jiu'an Agriculture Co.,Ltd.,Anyang 455000,Henan,China)

机构地区：[1]河南省纳普生物技术有限公司,郑州450002 [2]河南省生物技术开发中心,郑州450002 [3]河南省科学院,郑州450002 [4]安阳九安农业有限责任公司,河南安阳455000

出　　处：《中国油脂》2021年第10期127-131,共5页China Oils and Fats

基　　金：河南省科学院重大科研项目聚焦专项(190113004);河南省科学院基础科学研究项目(200613114)。

摘　　要：以亚麻荠籽饼为原料,以多糖得率为指标,在单因素试验基础上采用响应面试验对恒温搅拌提取亚麻荠籽多糖工艺进行优化,并探讨亚麻荠籽粗多糖提取物的体外抗氧化活性。结果表明,恒温搅拌提取亚麻荠籽多糖的最佳工艺条件为提取温度90℃、料液比1∶18、提取时间85 min、搅拌速度200 r/min,在此条件下提取2次,亚麻荠籽多糖得率为1.71%。亚麻荠籽粗多糖提取物对DPPH自由基与羟自由基均呈现出一定的清除能力,对羟自由基的清除能力优于DPPH自由基。研究结果为亚麻荠籽多糖的提取以及应用提供了理论依据。Taking Camelina sativa(L.)Crantz seeds cake as the raw material,the extraction technology of polysaccharides by constant temperature stirring was optimized by response surface methodology based on single factor experiment.The in vitro antioxidant activities of the extract were measured.The results showed that the optimal extraction conditions were obtained as follows∶extraction temperature 90℃,solid-liquid ratio 1∶18,extraction time 85 min and stirring speed 200 r/min.Under the optimal conditions,the polysaccharides yield was 1.71%when being extracted twice.The crude polysaccharide extract exhibited certain scavenging effects on DPPH·and·OH,but the effect on latter was stronger than the former.The results could provide theoretical basis for the extraction and application of polysaccharides of Camelina sativa(L.)Crantz seeds.

关 键 词：亚麻荠籽 多糖 提取 抗氧化 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ281[轻工技术与工程—食品科学与工程]