LncRNA-HOTAIR可调节脂肪间充质干细胞向成骨细胞的分化  

LncRNA-HOTAIR can regulate differentiation of adipose derived mesenchymal stem cells into osteoblasts

作　　者：黄涛[1] 贾志强 赵晓光[1] 王磊[1] 方丽萍 翟文静 翟莎菲 周永新[1] Huang Tao;Jia Zhiqiang;Zhao Xiaoguang;Wang Lei;Fang Liping;Zhai Wenjing;Zhai Shafei;Zhou Yongxin(First Affiliated Hospital of Xi’an Medical College,Xi’an 710077,Shaanxi Province,China;Second Affiliated Hospital of Henan University of Science and Technology,Luoyang 471000,Henan Province,China;Xi’an People’s Hospital,Xi’an 710068,Shaanxi Province,China;Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,Shaanxi Province,China;School of Stomatology,Xi’an Medical College,Xi’an 710021,Shaanxi Province,China)

机构地区：[1]西安医学院第一附属医院,陕西省西安市710077 [2]河南科技大学第二附属医院,河南省洛阳市471000 [3]西安市人民医院,陕西省西安市710068 [4]西安交通大学第二附属医院,陕西省西安市710061 [5]西安医学院口腔医学院,陕西省西安市710021

出　　处：《中国组织工程研究》2022年第25期3951-3955,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81801237),项目参与人:黄涛。

摘　　要：背景:已有证据表明,LncRNA-HOTAIR能调节骨髓间充质干细胞中成骨相关基因的表达,但其对脂肪间充质干细胞成骨分化的影响尚未见报道。目的:探讨LncRNA-HOTAIR对脂肪间充质干细胞成骨分化的影响。方法:体外培养Lewis大鼠脂肪间充质干细胞,转染lncRNA-NC、lncRNA-HOTAIR mimics、lncRNA-HOTAIR inhibitor并进行成骨诱导,成骨诱导第7天检测碱性磷酸酶活性,成骨诱导第14天进行茜素红染色。成骨诱导第6天,通过RT-PCR及Western blot检测成骨相关基因碱性磷酸酶、Runt相关转录因子2、骨钙素、骨桥蛋白mRNA和蛋白的表达。结果与结论:①转染LncRNA-HOTAIR mimic后,脂肪间充质干细胞中碱性磷酸酶活性及钙化程度显著降低,而转染LncRNA-HOTAIR inhibitor后则显著增加(P<0.05);②转染LncRNA-HOTAIR mimic后,脂肪间充质干细胞中碱性磷酸酶、Runt相关转录因子2、骨钙素和骨桥蛋白的表达显著降低(P<0.05),而转染LncRNA-HOTAIR inhibitor后则显著增加(P<0.05);③上述结果表明,LncRNA-HOTAIR能负向调控脂肪间充质干细胞的成骨分化。BACKGROUND:Evidence has shown that LncRNA-HOTAIR can regulate the expression of osteogenic related genes in bone marrow mesenchymal stem cells,but its effect on the osteogenic differentiation of adipose derived mesenchymal stem cells has not been reported.OBJECTIVE:To investigate the effect of LncRNA-HOTAIR on the osteogenic differentiation of adipose derived mesenchymal stem cells.METHODS:Adipose derived mesenchymal stem cells of Lewis rats were cultured in vitro.LncRNA-NC,lncRNA-HOTAIR mimics,and lncRNA-HOTAIR inhibitor were transfected into adipose derived mesenchymal stem cells and osteogenesis was induced.The alkaline phosphatase activity was detected on the 7^(th) day of osteogenic induction.Alizarin red staining was performed on the 14^(th) day of osteogenic induction.On the 6^(th) day of osteogenic induction,the expression levels of osteogenic-related genes alkaline phosphatase,Runt-related transcription factor 2,osteocalcin,and osteopontin mRNA and protein were detected by RT-PCR and western blot assay.RESULTS AND CONCLUSION:(1)After LncRNA-HOTAIR mimic transfection,alkaline phosphatase activity and calcification degree of adipose derived mesenchymal stem cells were significantly decreased,while LncRNA-HOTAIR inhibitor transfection significantly increased(P<0.05).(2)After LncRNA-HOTAIR mimic transfection,the expression of alkaline phosphatase,Runt-related transcription factor 2,osteocalcin,and osteopontin in adipose derived mesenchymal stem cells decreased significantly(P<0.05),but increased significantly after LncRNA-HOTAIR inhibitor transfection(P<0.05).(3)These results suggest that LncRNA-HOTAIR can negatively regulate the osteogenic differentiation of adipose derived mesenchymal stem cells.

关 键 词：干细胞 脂肪间充质干细胞 LncRNA-HOTAIR MIMIC INHIBITOR 成骨分化 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]