响应面法优化提取仿刺参中海参皂苷工艺  

Optimization of Microwave-Assisted Extraction Conditions for Saponins in Sea Cucumber Apostichopus japonicus by Response Surface Methodology

作　　者：刘桂英[1] 王旭达 葛坤 胡超魁[1] 吴金浩[1] 李爱[1] 郑杰[1] 周遵春[1] LIU Guiying;WANG Xuda;GE Kun;HU Chaokui;WU Jinhao;LI Ai;ZHENG Jie;ZHOU Zunchun(Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China;Dalian Ocean School,Dalian 116023,China)

机构地区：[1]辽宁省海洋水产科学研究院,辽宁大连116023 [2]大连海洋学校,辽宁大连116023

出　　处：《水产科学》2021年第6期810-817,共8页Fisheries Science

基　　金：国家重点研发计划项目(2018YFD0900105);辽宁省重点研发计划项目(2018228004);辽宁省海洋与渔业科研项目(201828,201824);大连市高层次人才项目(2018RD10);大连市科技创新基金资助项目(2020JJ27SN113).

摘　　要：通过Box-Benhken响应面法优化仿刺参中海参皂苷的提取工艺,为海参皂苷提取提供借鉴。以海参皂苷CladolosideA(CA)为评价指标,以溶剂体积分数、提取次数和提取时间为因素,采用单因素法和Box-Benhken响应面法相结合的方法优化仿刺参中海参皂苷CA的提取工艺参数,从而确定海参皂苷的提取工艺。试验结果显示,以海参皂苷CA的含量为指标测定提取液,优化的提取工艺条件为:微波功率为400W,溶剂为25mL70%乙醇,温度为45℃,次数为3次,时间为10min,在此条件下,海参皂苷CA的含量达151.8μg/g,与预测值相近。本试验采用的海参皂苷的提取方法操作简便,提取效率较高,为刺参皂苷的提取和利用提供科学依据。The process of saponin extracting from sea cucumber Apostichopus japonicus was optimized to provide reference for the quality evaluation.Cladoloside A was used for evaluation index,and three independent variable factors including microwave time,temperature and ethanol concentrations were examined.The extraction process of cladoloside A was optimized by single factor experiment coupled with Box-Benhken response surface method.Results showed that based on the regression result and considering the feasibility of experiment conduction,the following independent variable values were chosen as the optimized conditions for the extraction of saponin:extraction time of 10 min,extraction temperature of 45℃and 25 mL 70%ethanol under 400 W.The response surface method was used to optimize the extraction of saponins,and it was feasible to provide technical support for the quality control of sea cucumber.

关 键 词：仿刺参 海参皂苷 微波萃取 响应面 

分 类 号：S985.9[农业科学—捕捞与储运]