饲料投喂、饥饿与活饵再投喂对鳜幼鱼糖代谢的影响  

Effects of Feed Feeding,Starvation and Live Food Refeeding on Glucose Metabolism in Mandarinfish Siniperca chuatsi

作　　者：马晨夕 赵金良[1] 曾萌冬 宋银都 周云红 MA Chenxi;ZHAO Jinliang;ZENG Mengdong;SONG Yindu;ZHOU Yunhong(Laboratory of Freshwater Fisheries Germplasm Resource,Ministry of Agriculture and Rural Affairs,Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding,National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学,农业农村部淡水水产种质资源重点实验室,水产动物遗传育种中心上海市协同创新中心,水产科学国家级实验教学示范中心,上海201306

出　　处：《水产科学》2021年第6期818-825,共8页Fisheries Science

基　　金：财政部和农业农村部现代农业产业技术体系专项(CARS-46).

摘　　要：为探明营养水平变化对鳜鱼基础糖代谢的影响,对平均体质量分别为(3.27±0.63)、(3.54±0.52)g的鳜幼鱼进行为期3周的饲料投喂、饥饿与再投喂试验,比较测定了鳜幼鱼生长指标、肝糖原、肌糖原、血糖含量、胰岛素和胰高血糖素含量,肝脏中糖酵解、戊糖磷酸、糖异生等途径中9个代谢酶活性。试验结果显示,饲料组生长显著低于活饵组,两组间肝糖原、肌糖原、血糖以及胰岛素、胰高血糖素含量未见明显差异,除饲料组糖酵解途径中磷酸果糖激酶、丙酮酸激酶活性显著高于活饵组外,其他酶活性无显著变化。与正常投喂组相比,饥饿3周后,体质量、肝质量、肝指数显著下降;肝糖原、肌糖原、血糖含量显著下降;胰岛素含量下降,胰高血糖素含量升高;戊糖磷酸途径代谢酶葡萄糖脱氢酶活性下降最为显著,其次是糖酵解代谢酶磷酸果糖激酶、丙酮酸激酶;糖异生代谢酶磷酸烯醇丙酮酸羧化激酶、果糖-1,6-二磷酸酶活性未见明显变化。饥饿2周再投喂1周后,肝质量、肝指数恢复快于体质量恢复;血糖恢复快于肝糖原和肌糖原恢复;胰岛素、胰高血糖素含量恢复正常;各代谢酶活性均表现恢复。试验结果表明,饲料投喂对鳜幼鱼糖代谢无明显影响,而饥饿与再投喂对鳜幼鱼糖代谢影响较显著。In order to understand the effects of nutrient levels on glucose metabolism of mandarinfish Siniperca chuatsi,a three-week feed feeding experiment,and a starvation and refeeding experiment were carried out in juvenile with average body weights of(3.27±0.63)g and(3.54±0.52)g,and growth index,liver index,liver glycogen,muscle glycogen and blood glucose contents,insulin and glucagon contents,and 9 enzyme activities in glycolysis,pentose phosphate,gluconeogenesis pathways were measured and compared.The results showed that in feed feeding group there was significantly lower growth than that in the live food group,without significant difference in blood glucose,liver glycogen,muscle glycogen,insulin and glucagon contents between two groups,most enzymatic activities did not change significantly except for phosphofructokinase(PFK),and pyruvate kinase(PK).Compared with the normal feeding group,after three weeks of starvation,body weight,liver weight and liver index were significantly decreased.Liver glycogen and muscle glycogen,and blood glucose contents were significantly declined;insulin decreased and glucagon content increased;glucose dehydrogenase(GDH)activity decreased significantly,followed by PFK and PK activities,whereas phosphoenolpyruvate carboxykinase(PEPCK)and fructose-1,6-bisphosphatase(FBPase)activities did not changed.After two weeks of starvation and one week of refeeding,liver weight and liver index recovered faster than body weight.Blood glucose recovered faster than liver glycogen and muscle glycogen levels;insulin and glucagon levels were returned to normal level;all enzymatic activity restored.These findings indicated that feed feeding had no significant effect on glucose metabolism,while a significant change on glucose metabolism happened in starvation and refeeding of mandarinfish.

关 键 词：鳜鱼 血糖 糖原 糖代谢酶 饲料投喂 饥饿与再投喂 

分 类 号：S965.199[农业科学—水产养殖]