Light-up lipid droplets for the visualization of lipophagy and atherosclerosis by coumarin-derived bioprobe  

作　　者：Jinrong Zheng Shuheng Qin Lijuan Gui Hua Li Lixue Fan Yifei Yang Haiyan Chen Hui Xu Zhenwei Yuan 

机构地区：[1]Department of Biomedical Engineering,School of Engineering,China Pharmaceutical University,Nanjing 210009,China [2]School of Pharmacy,Key Laboratory of Molecular Pharmacology and Drug Evaluation(Yantai University),Ministry of Education,Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong,Yantai University,Yantai 264005,China [3]Department of Food Quality and Safety,College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing,Nanjing University of Finance and Economics,Nanjing 210023,China

出　　处：《Chinese Chemical Letters》2021年第8期2385-2389,共5页中国化学快报（英文版）

基　　金：National Natural Science Foundation of China (No.82001891);the Post-doctoral Innovative Talent Support Program (No.BX20190389);China Postdoctoral Science Foundation (No.2019M662009)。

摘　　要：Lipid droplets(LDs) are intracellular lipid-metabolism organelles that involved in many physiological processes,metabolic disorders as well as diseases such as atherosclerosis.However,the specific probes that can visually locate abnormal LDs-rich tissues and track LDs-associated behavior to the naked eye with adequate biosafety still are rare.Herein,we develop a new design strategy of LDs-targeted probe based on the solvatochromism of coumarin derivatives.The results revealed that the emission wavelength of coumarin fluorophores gradually red shift in different solvents with increasing polarity,while absorption wavelength almost unchanged.As a result,the enlarged stokes shift of coumarin was emerged from oil to water.Furthermore,properly reducing water solubility and adding electronic donor at the structure of coumarins can enlarge this type of solvatochromism.This discovery was utilized to develop suitable probe for the image of LDs and LDs-rich tissues with high resolution and biosafety.Therefore,LDs-associated behavior was visible to the naked eye during the process of lipophagy and atherosclerosis.We deem that the developed probe here offers a new possibility to accurately diagnosis and analyse LDs-related diseases in clinic and preclinical study.

关 键 词：Lipid droplets PROBE Lipophagy ATHEROSCLEROSIS 

分 类 号：R318[医药卫生—生物医学工程] O657.3[医药卫生—基础医学]