基于联合分析方法的二倍体马铃薯群体抗旱性评价  

Evaluation of Drought Resistance of Diploid Potato Population Based on Conjoint Analysis Method

作　　者：高春燕 秦军红[1] 徐建飞[1] 段绍光[1] 卞春松[1] 金黎平[1] 李广存[1] GAO Chun-yan;QIN Jun-hong;XU Jian-fei;DUAN Shao-guang;BIAN Chun-song;JIN Li-ping;LI Guang-cun(Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop,Ministry of Agriculture and Rural Affairs/Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081)

机构地区：[1]农业农村部薯类作物生物学和遗传育种重点实验室/中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《植物遗传资源学报》2021年第6期1542-1549,共8页Journal of Plant Genetic Resources

基　　金：宁夏回族自治区农业育种专项(2019NYYZ01-1);国家马铃薯产业技术体系项目(CARS-9)。

摘　　要：马铃薯是世界第四大粮食作物,其在应对世界粮食危机和营养不良等问题方面具有重要作用。马铃薯属于浅根系作物,我国马铃薯主要种植在干旱半干旱地区,其生长受干旱影响显著。因此,培育抗旱品种对保证旱区马铃薯产量、提高水资源利用率具有重要意义。本研究分别在2019-2020年于网棚、大田、抗旱棚3个环境设正常灌溉和水分亏缺2个水分处理,在收获时分别测定各处理下二倍体马铃薯群体各基因型的产量并计算抗旱系数,通过差异显著性分析、相关性分析、方差分析和双标图分析等联合分析了该群体在3个环境下的抗旱性,结果表明:2020年大田试验和2020年抗旱棚试验反映了群体各基因型在较轻程度干旱胁迫下的抗旱能力;2019年网棚试验反映了群体各基因型在较重程度干旱胁迫下的抗旱能力。在2种不同干旱胁迫程度下抗旱等级均一致的基因型有58个,占群体总数的47.93%。本研究为马铃薯群体重复多次的抗旱性评价提供了一定的理论依据;筛选出的抗旱性材料可为后期研究马铃薯抗旱关键基因的定位、克隆及抗旱机制的解析奠定坚实的基础。Potato is ranking the world′s fourth largest food crop which palyed an important role in ensuring global food crisis and malnutrition.Potatoes are shallow-rooted crops and sensitive to drought stress which are cultivated in arid and semi-arid areas in China.Breeding drought-resistant varieties is thus of great significance to ensure potato yield and improve water resource utilization in arid areas.In this study,two treatments of normal irrigation and deficit irrigation were set up,and the yield of each genotype of diploid potato population under different treatment was measured at harvest and the drought resistance coefficient was calculated.At harvest,the yield of each genotype of diploid potato population under each treatment was measured and the drought resistance coefficient was calculated,and the difference of the drought resistance of this population in three environments was analyzed by jointly with significance analysis,correlation analysis,and variance analysis and double plot analysis and so on.The results showed that the field experiment in 2020 and the drought-resistant greenhouse experiment in 2020 were suitable to reveal the drought resistance under mild drought stress conditions;the net greenhouse experiment in 2019 was suitable to reveal the drought resistance under severe drought stress condition.There were 58 genotypes with the same drought resistance level under the two different drought stress levels,accounting for 47.93%of the total population.The results provided certain theoretical basis for potato drought tolerance selection with several replications and the identified drought-resistant potato genoptyes which laid a foundation for future research on isolation of important genes and dissection of drought resistance mechanisms.

关 键 词：马铃薯 二倍体 抗旱性 评价 联合分析 

分 类 号：S532[农业科学—作物学]